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Planta

, Volume 242, Issue 1, pp 1–22 | Cite as

Symbiotic in vitro seed propagation of Dendrobium: fungal and bacterial partners and their influence on plant growth and development

  • Jaime A. Teixeira da SilvaEmail author
  • Elena A. TsavkelovaEmail author
  • Songjun ZengEmail author
  • Tzi Bun NgEmail author
  • S. ParthibhanEmail author
  • Judit DobránszkiEmail author
  • Jean Carlos CardosoEmail author
  • M. V. RaoEmail author
Review

Abstract

The genus Dendrobium is one of the largest genera of the Orchidaceae Juss. family, although some of its members are the most threatened today. The reason why many species face a vulnerable or endangered status is primarily because of anthropogenic interference in natural habitats and commercial overexploitation. The development and application of modern techniques and strategies directed towards in vitro propagation of orchids not only increases their number but also provides a viable means to conserve plants in an artificial environment, both in vitro and ex vitro, thus providing material for reintroduction. Dendrobium seed germination and propagation are challenging processes in vivo and in vitro, especially when the extreme specialization of these plants is considered: (1) their biotic relationships with pollinators and mycorrhizae; (2) adaptation to epiphytic or lithophytic life-styles; (3) fine-scale requirements for an optimal combination of nutrients, light, temperature, and pH. This review also aims to summarize the available data on symbiotic in vitro Dendrobium seed germination. The influence of abiotic factors as well as composition and amounts of different exogenous nutrient substances is examined. With a view to better understanding how to optimize and control in vitro symbiotic associations, a part of the review describes the strong biotic relations of Dendrobium with different associative microorganisms that form microbial communities with adult plants, and also influence symbiotic seed germination. The beneficial role of plant growth-promoting bacteria is also discussed.

Keywords

Dendrobium germination and propagation Mycorrhiza Orchid-associated bacteria Orchidaceae Symbiotic seed germination 

Abbreviations

ABA

Abscisic acid

AMF

Arbuscular mycorrhizal fungi

C

Carbon

CMA

Cornmeal agar

DW

Dry weight

FW

Fresh weight

GA

Gibberellin

GA3

Gibberellic acid

IAA

Indole-3-acetic acid

ITS

Internal transcribed spacer

MH

Mycoheterotrophy

N

Nitrogen

OMA

Oatmeal agar

OMF

Orchid mycorrhizal fungi

PGPR

Plant growth-promoting rhizobacteria

PGR

Plant growth regulator

PLB

Protocorm-like body

SEM

Scanning electron microscopy

Trp

Tryptophan

Notes

Acknowledgments

The authors thank Dr. Meesawat Upatham (Prince of Songkla University, Thailand) for comments and opinions on an earlier version of the manuscript.

Conflict of interest

The authors declare no conflicts of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Kagawa-KenJapan
  2. 2.Department of Microbiology, Faculty of BiologyLomonosov Moscow State UniversityMoscowRussia
  3. 3.Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical GardenChinese Academy of SciencesGuangzhouChina
  4. 4.School of Biomedical Sciences, Faculty of MedicineThe Chinese University of Hong KongHong KongChina
  5. 5.Shenzhen Research InstituteThe Chinese University of Hong KongShenzhenChina
  6. 6.Department of Plant ScienceBharathidasan UniversityTiruchirappalliIndia
  7. 7.Research Institute of NyíregyházaUniversity of DebrecenNyíregyházaHungary
  8. 8.Department of Rural DevelopmentCentro de Ciências Agrárias, UFSCarArarasBrazil

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