Dynamics of flavonol accumulation in leaf tissues under different UV-B regimes in Centella asiatica (Apiaceae)

Abstract

Main conclusion

A cumulative effect of UV-B doses on epidermal flavonol accumulation was observed during the first week of a time course study in Centella asiatica (Apiaceae). However, once flavonol levels had peaked, additional accumulation was possible only if higher daily UV-B irradiances were applied.

We aimed to understand the dynamics of flavonol accumulation in leaf tissues using non-destructive spectroscopy and HPLC–mass spectrometry. When leaves that had grown without UV-B were given brief daily exposures to low-irradiance UV-B, they accumulated flavonols, predominantly kaempferol-3-O-β-d-glucuronopyranoside and quercetin-3-O-β-d-glucuronopyranoside, in their exposed epidermis, reaching a plateau after 7 days. More prolonged UV-B exposures or higher doses eventually augmented flavonol concentrations even in non-exposed tissues. If UV-B irradiance was subsequently reduced, leaves appeared to lose their ability to accumulate further flavonols in their epidermis even if the duration of daily exposure was increased. A higher irradiance level was then necessary to further increase flavonol accumulation. When subsequently acclimated to higher UV-B irradiances, mature leaves accumulated less flavonols than did developing ones. Our study suggests that levels of epidermal flavonols in leaves are governed primarily by UV-B irradiance rather than by duration of exposure.

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Abbreviations

Amu:

Atomic mass unit

AU:

Absorbance units

ChlF:

Chlorophyll a fluorescence

ChlF(ex375), (ex655),:

Chlorophyll a fluorescence induced by 375 nm or by 655 nm excitation wavelength

COP1 :

CONSTITUTIVELY PHOTOMORPHOGENIC1 gene

DPBA:

Diphenyl boric acid-2-aminoethyl ester

DA375 :

Dualex absorbance at 375 nm

LC–MS:

Liquid chromatography–mass spectrometry

PAR:

Photosynthetically active radiation

ROS:

Reactive oxygen species

SIR:

Single-ion record

RUP1:

REPRESSOR OF UV-B PHOTOMORPHOGENESIS1 protein

RUP2:

REPRESSOR OF UV-B PHOTOMORPHOGENESIS2 protein

UVR8 :

UV RESISTANCE LOCUS8 gene

UV-A:

Ultraviolet-A radiation (315–400 nm)

UV-B:

Ultraviolet-B radiation (280–315 nm)

SAA:

Systemic acquired acclimation

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Acknowledgments

We thank the Sotramex Company (Madagascar), which kindly provided Centella asiatica from Madagascar. This work was funded by the bilateral contract BISCAP 4 & 5 no 130913 between Bayer HealthCare and University of Montpellier during the period 2008–2013.

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Correspondence to Luc P. R. Bidel.

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Special topic: Polyphenols: biosynthesis and function in plants and ecosystems. Guest editor: Stefan Martens.

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Bidel, L.P.R., Chomicki, G., Bonini, F. et al. Dynamics of flavonol accumulation in leaf tissues under different UV-B regimes in Centella asiatica (Apiaceae). Planta 242, 545–559 (2015). https://doi.org/10.1007/s00425-015-2291-7

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Keywords

  • Biospectroscopy
  • Centella asiatica
  • Dualex
  • Light acclimation
  • Polyphenols
  • UV-B absorption