Planta

, Volume 241, Issue 4, pp 823–836 | Cite as

Isolation and characterization of an ubiquitin extension protein gene (JcUEP) promoter from Jatropha curcas

  • Yan-Bin Tao
  • Liang-Liang He
  • Long-Jian Niu
  • Zeng-Fu Xu
Original Article

Abstract

Main conclusion

TheJcUEPpromoter is active constitutively in the bio-fuel plantJatrophacurcas, and is an alternative to the widely usedCaMV35Spromoter for driving constitutive overexpression of transgenes inJatropha.

Well-characterized promoters are required for transgenic breeding of Jatropha curcas, a biofuel feedstock with great potential for production of bio-diesel and bio-jet fuel. In this study, an ubiquitin extension protein gene from Jatropha, designated JcUEP, was identified to be ubiquitously expressed. Thus, we isolated a 1.2 kb fragment of the 5′ flanking region of JcUEP and evaluated its activity as a constitutive promoter in Arabidopsis and Jatropha using the β-glucuronidase (GUS) reporter gene. As expected, histochemical GUS assay showed that the JcUEP promoter was active in all Arabidopsis and Jatropha tissues tested. We also compared the activity of the JcUEP promoter with that of the cauliflower mosaic virus 35S (CaMV35S) promoter, a well-characterized constitutive promoter conferring strong transgene expression in dicot species, in various tissues of Jatropha. In a fluorometric GUS assay, the two promoters showed similar activities in stems, mature leaves and female flowers; while the CaMV35S promoter was more effective than the JcUEP promoter in other tissues, especially young leaves and inflorescences. In addition, the JcUEP promoter retained its activity under stress conditions in low temperature, high salt, dehydration and exogenous ABA treatments. These results suggest that the plant-derived JcUEP promoter could be an alternative to the CaMV35S promoter for driving constitutive overexpression of transgenes in Jatropha and other plants.

Keywords

Physic nut Constitutive promoter Ubiquitin Transgenic Stress CaMV35S 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Yan-Bin Tao
    • 1
    • 2
  • Liang-Liang He
    • 1
  • Long-Jian Niu
    • 1
    • 3
  • Zeng-Fu Xu
    • 1
  1. 1.Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMenglunChina
  2. 2.University of Chinese Academy of SciencesBeijingChina
  3. 3.School of Life SciencesUniversity of Science and Technology of ChinaHefeiChina

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