Comparative proteomic analysis reveals the role of hydrogen sulfide in the adaptation of the alpine plant Lamiophlomis rotata to altitude gradient in the Northern Tibetan Plateau
We found the novel role of hydrogen sulfide in the adaptation of the alpine plant to altitude gradient in the Northern Tibetan Plateau.
Alpine plants have developed strategies to survive the extremely cold conditions prevailing at high altitudes; however, the mechanism underlying the evolution of these strategies remains unknown. Hydrogen sulfide (H2S) is an essential messenger that enhances plant tolerance to environmental stress; however, its role in alpine plant adaptation to environmental stress has not been reported until now. In this work, we conducted a comparative proteomics analysis to investigate the dynamic patterns of protein expression in Lamiophlomis rotata plants grown at three different altitudes. We identified and annotated 83 differentially expressed proteins. We found that the levels and enzyme activities of proteins involved in H2S biosynthesis markedly increased at higher altitudes, and that H2S accumulation increased. Exogenous H2S application increased antioxidant enzyme activity, which reduced ROS (reactive oxygen species) damage, and GSNOR (S-nitrosoglutathione reductase) activity, which reduced RNS (reactive nitrogen species) damage, and activated the downstream defense response, resulting in protein degradation and proline and sugar accumulation. However, such defense responses could be reversed by applying H2S biosynthesis inhibitors. Based on these findings, we conclude that L. rotata uses multiple strategies to adapt to the alpine stress environment and that H2S plays a central role during this process.
KeywordsAdaptation H2S Lamiophlomis Proteomics
Cysteine synthesis complex
Cystathionine c lyase
False discovery rates
Reactive oxygen species
Reactive nitrogen species
We thank the members of Yang Yongping’s group for their help during sample collection. This work was supported by the Young Academic and Technical Leader Raising Foundation of Yunnan Province (No. 2012HB041), the project of innovation team of Yunnan Province, the National Natural Sciences Foundation of China (No. 31170256), and the Major State Basic Research Development Program (2010CB951700).
- Hare PD, Cress WA, van Staden J (1999) Proline synthesis and degradation: a model system for elucidating stress-related signal transduction. J Exp Bot 50:413–434Google Scholar
- Herppich WB, Herppich M (1996) Ecophysiological investigations on plants of the genus Plectranthus (fam Lamiaceae) native to Yemen and southern Africa. Flora 191:401–408Google Scholar
- Stone SL (2014) The role of ubiquitin and the 26S proteasome in plant abiotic stress signaling. Frontiers Plant Sci 5: 135; doi: 10.3389/fpls.2014.00135