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Phenotyping carrot (Daucus carota L.) for yield-determining temperature response by calorespirometry

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Abstract

Main conclusion

Calorespirometric measurements proved to be useful for phenotyping temperature response in terms of optimum temperatures for growth and low temperature limits for growth respiration in diverse carrot genotypes.

High and low-temperature tolerance is an important trait in many breeding programs, but to date, improvement strategies have had limited success. Developing new, cost efficient and reliable screening tools to identify and select the most tolerant crop plant genotypes is necessary to assist plant breeding on cold and heat tolerance, and calorespirometry is proposed for this. Calorespirometry is a technique to simultaneously measure metabolic heat rates and CO2 emission rates of respiring tissues and can be used as a rapid method to determine how changes in the environment (e.g., temperature) influence plant growth. The main aim of this work was, therefore, to test the usefulness of calorespirometry as a phenotyping tool for carrot taproot growth in response to temperature. Calorespirometric measurements in the carrot taproot meristems of plants from eight carrot inbred lines allowed identification of optimum and minimum temperatures for growth of plants and to distinguish between phenotypes based on those characteristics. The technique proved to be useful for predicting yield-determining temperature responses in diverse carrot genotypes. Preliminary screening of new crop plant genotypes with calorespirometry based on their temperature adaptation and acclimation capability could make the screening process much less laborious by allowing selection of genotypes presenting the best growth performance under particular biotic or abiotic conditions before field tests.

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Acknowledgments

Authors want to acknowledge Dr Phil Simon from the Vegetable Crops Research Unit of the USDA-ARS service for providing the seeds of the carrot inbred lines used in this study and for providing the information about them, also to José Lourenço Brito e Abreu, Produçâo e Comercializaçâo de MicroLeafs, for providing the facilities for carrot growth experimentation. This work is funded by FEDER funds through the Operational Program for Competitiveness Factors—(COMPETE), national funds through FCT (Foundation for Science and Technology) under the strategic project (PEst-C/AGR/UI0115/2011), and the (PTDC/AGR-GPL/111196/2009) FCT project. The authors would like to thank to FCT for the support given under the program POPH—Programa Operacional Potencial Humano (Ciência 2007).

Conflict of interest

The authors declare that they have no conflict of interest.

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Author notes

  1. Luz Muñoz-Sanhueza

    Present address: Department of Plant Sciences (IPV), Norwegian University of Life Sciences (NMBU), P.O. Box 5003, 1432, Ås, Norway

Authors and Affiliations

  1. EU Marie Curie Chair, ICAAM - Instituto de Ciências Agrárias e Ambientais Mediterrânicas, IIFA-Instituto de Formação e Investigação Avançada, Universidade de Évora, Núcleo da Mitra, Ap. 94, 7002-554, Évora, Portugal

    Amaia Nogales & Birgit Arnholdt-Schmitt

  2. Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, 84602, USA

    Lee D. Hansen

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  1. Amaia Nogales
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Correspondence to Amaia Nogales.

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Nogales, A., Muñoz-Sanhueza, L., Hansen, L.D. et al. Phenotyping carrot (Daucus carota L.) for yield-determining temperature response by calorespirometry. Planta 241, 525–538 (2015). https://doi.org/10.1007/s00425-014-2195-y

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