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Planta

, Volume 241, Issue 1, pp 57–67 | Cite as

Spatial and temporal activity of the foxtail millet (Setaria italica) seed-specific promoter pF128

  • Yanlin Pan
  • Xin Ma
  • Hanwen Liang
  • Qian Zhao
  • Dengyun Zhu
  • Jingjuan Yu
Original Article

Abstract

Main conclusion

pF128 drives GUS specifically expressed in transgenic seeds of foxtail millet and Zea mays with higher activity than the constitutive CaMV35S promoter and the maize seed-specific 19Z promoter.

Abstract

Foxtail millet (Setaria italica), a member of the Poaceae family, is an important food and fodder crop in arid regions. Foxtail millet is an excellent C4 crop model owing to its small genome (~490 Mb), self-pollination and availability of a complete genome sequence. F128 was isolated from a cDNA library of foxtail millet immature seeds. Real-time PCR analysis revealed that F128 mRNA was specifically expressed in immature and mature seeds. The highest F128 mRNA level was observed 5 days after pollination and gradually decreased as the seed matured. Sequence analysis suggested that the protein encoded by F128 is likely a protease inhibitor/seed storage protein/lipid-transfer protein. The 1,053 bp 5′ flanking sequence of F128 (pF128) was isolated and fused to the GUS reporter gene. The corresponding vector was then transformed into Arabidopsis thaliana, foxtail millet and Zea mays. GUS analysis revealed that pF128 drove GUS expression efficiently and specifically in the seeds of transgenic Arabidopsis, foxtail millet and Zea mays. GUS activity was also detected in Arabidopsis cotyledons. Activity of pF128 was higher than that observed for the constitutive CaMV35S promoter and the maize seed-specific 19 Zein (19Z) promoter. These results indicate that pF128 is a seed-specific promoter. Its application is expected to be of considerable value in plant genetic engineering.

Keywords

Foxtail millet Lipid-transfer proteins Promoter Embryo Endosperm transfer cell 

Notes

Acknowledgments

This work was supported by the National Transgenic Major Program of China (grant no. 2008ZX08003-002, 2009ZX08009093-002, 2011ZX08003-002 and 2013ZX08003-002).

Supplementary material

425_2014_2164_MOESM1_ESM.docx (960 kb)
Supplementary material 1 (DOCX 960 kb)
425_2014_2164_MOESM2_ESM.pdf (106 kb)
Supplementary material 2 (PDF 106 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Yanlin Pan
    • 1
  • Xin Ma
    • 1
  • Hanwen Liang
    • 1
  • Qian Zhao
    • 1
  • Dengyun Zhu
    • 1
  • Jingjuan Yu
    • 1
  1. 1.State Key Laboratory of Agrobiotechnology, College of Biological SciencesChina Agricultural UniversityBeijingChina

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