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The APX4 locus regulates seed vigor and seedling growth in Arabidopsis thaliana

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Abstract

The amino acid sequence of APX4 is similar to other ascorbate peroxidases (APXs), a group of proteins that protect plants from oxidative damage by transferring electrons from ascorbate to detoxify peroxides. In this study, we characterized two apx4 mutant alleles. Translational fusions with GFP indicated APX4 localizes to chloroplasts. Both apx4 mutant alleles formed chlorotic cotyledons with significantly reduced chlorophyll a, chlorophyll b and lutein. Given the homology of APX to ROS-scavenging proteins, this result is consistent with APX4 protecting seedling photosystems from oxidation. The growth of apx4 seedlings was stunted early in seedling development. In addition, APX4 altered seed quality by affecting seed coat formation. While apx4 seed development appeared normal, the seed coat was darker and more permeable than the wild type. In addition, accelerated aging tests showed that apx4 seeds were more sensitive to environmental stress than the wild-type seeds. If APX4 affects seed pigment biosynthesis or reduction, the seed coat color and permeability phenotypes are explained. apx4 mutants had cotyledon chlorosis, increased H2O2 accumulation, and reduced soluble APX activity in seedlings. These results indicate that APX4 is involved in the ROS-scavenging process in chloroplasts.

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Abbreviations

APX:

Ascorbate peroxidase

CC:

Chlorotic cotyledons

CCP:

Cytochrome c peroxidase

GC:

Green cotyledons

GFP:

Green fluorescent protein

GR:

Glutathione reductase

GUS:

β-Glucuronidase

H2O2 :

Hydrogen peroxide

MDAR/MDHAR:

Monodehydroascorbate reductase

PCR:

Polymerase chain reaction

SOD:

Superoxide dismutase

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Acknowledgments

We thank Dr. Ning-Hui Cheng in Baylor College of Medicine for the gift of the DsRed-KSRM construct and reviewers for constructive comments. This research was supported by the National Research Initiative of the USDA Cooperative State Research, Education, and Extension Service (grant number 2008-35100-19244).

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  1. Department of Biology, University of Florida, 220 Bartram Hall, Gainesville, 32611, USA

    Ya-Ying Wang, Amanda G. Hecker & Bernard A. Hauser

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  1. Ya-Ying Wang
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  2. Amanda G. Hecker
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  3. Bernard A. Hauser
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Correspondence to Bernard A. Hauser.

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Wang, YY., Hecker, A.G. & Hauser, B.A. The APX4 locus regulates seed vigor and seedling growth in Arabidopsis thaliana . Planta 239, 909–919 (2014). https://doi.org/10.1007/s00425-014-2025-2

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