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A novel transcription factor JcNAC1 response to stress in new model woody plant Jatropha curcas

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Abstract

Jatropha curcas, a biodiesel plant with a short life cycle, has great potentials to be a new model woody plant. In this study, we found a plant-specific transcription factor JcNAC1, an intriguing regulator modulating plant responses to abiotic stresses and pathogen infection. Expression of JcNAC1 was strongly increased when plants were treated with abscisic acid, salt and polyethylene glycol, and was decreased with salicylic acid, ethylene, and pathogens. Overexpressing JcNAC1 plants showed enhanced tolerance to drought and increased susceptibility to pathogens. Furthermore, over-expression of JcNAC1 in plants also resulted in the expression changes of some stress-related maker genes including curcin-L, which is a special stress-inducible ribosome-inactivating protein gene in J. curcas. These results indicate that JcNAC1 is responsible for stress responses in J. curcas.

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Abbreviations

ABA:

Abscisic acid

PEG:

Polyethylene glycol

SA:

Salicylic acid

MeJA:

Methyl jasmonate

GUS:

β-Glucuronidase

GFP:

Green fluorescent protein

NACRS:

NAC recognition sequence

ROS:

Reactive oxygen species

Pst DC3000:

Pseudomonas syringae pv. tomato DC3000

JAS:

JcNAC1 amiRNA silencing Jatropha curcas

OE:

JcNAC1 over-expressing Jatropha curcas

CLS:

Curcin-L silencing Jatropha curcas

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Acknowledgments

We thank H. Jin (Department of Plant Pathology and Microbiology, University of California, Riverside, CA, USA) and J. K. Zhu (Department of Botany and Plant Sciences, University of California, Riverside, CA, USA) for technical assistance in their labs. We also thank the CSC’s support. This work was funded by grants from the “12th Five-Year Plan” to support science and technology project (No. 2011BAD22B08).

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Authors and Affiliations

  1. Department of Plant Pathology and Microbiology, University of California, Riverside, CA, 92521, USA

    Xiaobo Qin & Yifan Lii

  2. College of Life Sciences, Sichuan University, Chengdu, 610064, China

    Xiaobo Qin, Xiaojiang Zheng, Caixia Shao, Ying Xu & Fang Chen

  3. Sichuan Natural Resource Institute, Chengdu, 610015, China

    Xiaobo Qin

  4. Guangzhou University of Chinese Medicine, Guangzhou, 510405, China

    Xiaoqi Huang

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  1. Xiaobo Qin
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Correspondence to Xiaobo Qin.

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X. Qin and X. Zheng contributed equally to the work.

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Qin, X., Zheng, X., Huang, X. et al. A novel transcription factor JcNAC1 response to stress in new model woody plant Jatropha curcas . Planta 239, 511–520 (2014). https://doi.org/10.1007/s00425-013-1993-y

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