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Pepper osmotin-like protein 1 (CaOSM1) is an essential component for defense response, cell death, and oxidative burst in plants

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Abstract

Osmotin or osmotin-like protein, a PR-5 family member, is differentially induced in plants by abiotic and biotic stresses. Here, we demonstrate that the pepper (Capsicum annuum) osmotin-like protein 1 gene, CaOSM1, was required for the defense and hypersensitive cell death response and oxidative burst signaling during Xanthomonas campestris pv. vesicatoria (Xcv) infection. CaOSM1 protein was localized to the plasma membrane in leaf cells of Nicotiana benthamiana. Agrobacterium-mediated transient expression of CaOSM1 in pepper distinctly induced the hypersensitive cell death response and H2O2 accumulation. Knock-down of CaOSM1 in pepper by virus-induced gene silencing increased the susceptibility to Xcv infection, which was accompanied by attenuation of the cell death response and decreased accumulation of H2O2. CaOSM1 overexpression in transgenic Arabidopsis conferred reduced susceptibility and accelerated cell death response and H2O2 accumulation to infection by Pseudomonas syringe pv. tomato and Hyaloperonospora arabidopsidis. Together, these results suggest that CaOSM1 is involved in cell death and oxidative burst responses during plant defense against microbial pathogens.

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Acknowledgments

This work was supported by the Next Generation BioGreen21 Program (Plant Molecular Breeding Center, Grant no. PJ008027 and Agricultural Genome Center, Grant no. PJ008211) of the Rural Development Administration, Republic of Korea.

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  1. Du Seok Choi

    Present address: Department of Plant Biology and The Genome Center, College of Biological Sciences, University of California Davis, Davis, CA, 95616, USA

Authors and Affiliations

  1. Laboratory of Molecular Plant Pathology, College of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-ku, Seoul, 136-713, Republic of Korea

    Du Seok Choi & Byung Kook Hwang

  2. Laboratory of Plant Pathology and Protection, Department of Horticulture, Gyeongnam National University of Science and Technology, Chilam-dong, Jinju, 660-758, Republic of Korea

    Jeum Kyu Hong

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  1. Du Seok Choi
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Correspondence to Byung Kook Hwang.

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Choi, D.S., Hong, J.K. & Hwang, B.K. Pepper osmotin-like protein 1 (CaOSM1) is an essential component for defense response, cell death, and oxidative burst in plants. Planta 238, 1113–1124 (2013). https://doi.org/10.1007/s00425-013-1956-3

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