Abstract
We investigated the relation between auxin-induced gene expression and the rapid auxin-induced growth inhibition in Arabidopsis thaliana roots. The natural auxin indole-3-acetic acid (IAA) induced a strong activation of gene expression as visualized by the DR5rev::GFP reporter gene technique. This effect was specific for active auxins and was abolished in knockout mutants of the F-box auxin receptors. We measured the IAA-induced growth inhibition at high time resolution and show that the F-box auxin receptor mutants failed to display this effect. We conclude that the F-box auxin receptors are needed for the response. In hypocotyls, auxin induces an increase in elongation growth, and this effect has been earlier shown to be independent of the F-box receptors. Based on these findings, we discuss differences in the growth control modes in roots and shoots. We demonstrate that the rapid auxin-induced root growth inhibition, unlike the induction of growth in hypocotyls, requires the presence of the F-box auxin receptors.
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Abbreviations
- AFB:
-
Auxin receptor F-box proteins
- TIR1:
-
Transport inhibitor response
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Acknowledgments
We thank Kjeld Engvild (Risø National Laboratory, Roskilde, Denmark) for providing samples of 4-Cl-IAA and 5,7-Cl2-IAA and Mark Estelle, University of California at San Diego (USA) for the mutant lines, and Julia Borgward (University of Hamburg) for helpful discussion.
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We declare no conflict of interests.
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Scheitz, K., Lüthen, H. & Schenck, D. Rapid auxin-induced root growth inhibition requires the TIR and AFB auxin receptors. Planta 238, 1171–1176 (2013). https://doi.org/10.1007/s00425-013-1941-x
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DOI: https://doi.org/10.1007/s00425-013-1941-x