Planta

, Volume 237, Issue 6, pp 1585–1597

An integrative analysis of four CESA isoforms specific for fiber cellulose production between Gossypium hirsutum and Gossypium barbadense

  • Ao Li
  • Tao Xia
  • Wen Xu
  • Tingting Chen
  • Xianliang Li
  • Jian Fan
  • Ruyi Wang
  • Shengqiu Feng
  • Yanting Wang
  • Bingrui Wang
  • Liangcai Peng
Original Article

DOI: 10.1007/s00425-013-1868-2

Cite this article as:
Li, A., Xia, T., Xu, W. et al. Planta (2013) 237: 1585. doi:10.1007/s00425-013-1868-2

Abstract

Cotton fiber is an excellent model system of cellulose biosynthesis; however, it has not been widely studied due to the lack of information about the cellulose synthase (CESA) family of genes in cotton. In this study, we initially identified six full-length CESA genes designated as GhCESA5–GhCESA10. Phylogenetic analysis and gene co-expression profiling revealed that CESA1, CESA2, CESA7, and CESA8 were the major isoforms for secondary cell wall biosynthesis, whereas CESA3, CESA5, CESA6, CESA9, and CESA10 should involve in primary cell wall formation for cotton fiber initiation and elongation. Using integrative analysis of gene expression patterns, CESA protein levels, and cellulose biosynthesis in vivo, we detected that CESA8 could play an enhancing role for rapid and massive cellulose accumulation in Gossypium hirsutum and Gossypium barbadense. We found that CESA2 displayed a major expression in non-fiber tissues and that CESA1, a housekeeping gene like, was predominantly expressed in all tissues. Further, a dynamic alteration was observed in cell wall composition and a significant discrepancy was observed between the cotton species during fiber elongation, suggesting that pectin accumulation and xyloglucan reduction might contribute to cell wall transition. In addition, we discussed that callose synthesis might be regulated in vivo for massive cellulose production during active secondary cell wall biosynthesis in cotton fibers.

Keywords

Cellulose CESA Cotton fiber Secondary cell wall biosynthesis 

Abbreviations

CalS

Callose synthase

CESA

Cellulose synthase

CrI

Crystalline index

CSC

Cellulose synthase complex

DP

Degree of polymerization

EST

Expression sequence tag

Gb

Gossypium barbadense

Gh

Gossypium hirsutum

Kor

Korrigan

RACE

Rapid-amplification of cDNA ends

SGT

Sterol glycosyltransferase

UBQ

Ubiquitin

Supplementary material

425_2013_1868_MOESM1_ESM.pdf (1.2 mb)
Supplementary material 1 (PDF 1193 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Ao Li
    • 1
    • 2
    • 3
  • Tao Xia
    • 1
    • 2
    • 4
  • Wen Xu
    • 1
    • 2
    • 4
  • Tingting Chen
    • 1
    • 2
    • 4
  • Xianliang Li
    • 1
    • 2
    • 3
  • Jian Fan
    • 1
    • 2
    • 3
  • Ruyi Wang
    • 1
    • 2
    • 3
  • Shengqiu Feng
    • 1
    • 2
    • 3
  • Yanting Wang
    • 1
    • 2
    • 3
  • Bingrui Wang
    • 3
  • Liangcai Peng
    • 1
    • 2
    • 3
    • 4
  1. 1.National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan)Huazhong Agricultural UniversityWuhanChina
  2. 2.Biomass and Bioenergy Research CentreHuazhong Agricultural UniversityWuhanChina
  3. 3.College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina
  4. 4.College of Life Science and TechnologyHuazhong Agricultural UniversityWuhanChina

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