, Volume 237, Issue 3, pp 771–781 | Cite as

Comparative analysis of barley leaf proteome as affected by drought stress

  • Ahmed AshoubEmail author
  • Tobias Beckhaus
  • Thomas Berberich
  • Michael Karas
  • Wolfgang Brüggemann
Original Article


The adaptive response of Egyptian barley land races to drought stress was analyzed using difference gel electrophoresis (DIGE). Physiological measurements and proteome alterations of accession number 15141, drought tolerant, and accession number 15163, drought sensitive, were compared. Differentially expressed proteins were subjected to MALDI-TOF-MS analysis. Alterations in proteins related to the energy balance and chaperons were the most characteristic features to explain the differences between the drought-tolerant and the drought-sensitive accessions. Further alterations in the levels of proteins involved in metabolism, transcription and protein synthesis are also indicated.


Barley Difference gel electrophoresis (DIGE) Drought stress Fv/Fm MALDI-TOF-MS Performance index Proteome analysis 



Two-dimensional polyacrylamide gel electrophoresis


Difference gel electrophoresis


Heat shock protein


Isoelectric focusing


Leaf relative water content


Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry


Plant efficiency analyzer


Performance index


Protein disulfide isomerase



The authors would like to thank the Egyptian National Gene Bank, Agricultural Research Center, for providing the seed material of the accession numbers used in this study. We would like to thank Prof. Dr. Gharib A. Gad EL-Karim at Department of Bioinformatics and Networks, AGERI, for helping in the statistical analysis carried out in this study. This study was funded by the research funding program “LOEWE—Landes-Offensive zur Entwicklung Wissenschaftlich-ökonomischer Exzellenz” of Hesse’s Ministry of Higher Education, Research, and the Arts.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Ahmed Ashoub
    • 1
    • 2
    • 4
    Email author
  • Tobias Beckhaus
    • 3
  • Thomas Berberich
    • 1
  • Michael Karas
    • 3
  • Wolfgang Brüggemann
    • 1
    • 4
  1. 1.Biodiversity and Climate Research Centre (BiK-F)Frankfurt am MainGermany
  2. 2.Agricultural Genetic Engineering Research Institute (AGERI)ARCGizaEgypt
  3. 3.Institute of Pharmaceutical ChemistryJohann Wolfgang Goethe-University FrankfurtFrankfurt am MainGermany
  4. 4.Institute of Ecology, Evolution, and DiversityJohann Wolfgang Goethe-University FrankfurtFrankfurt am MainGermany

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