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Arabidopsis thaliana Oxa proteins locate to mitochondria and fulfill essential roles during embryo development

Abstract

Members of the Alb3/Oxa1/YidC protein family function as insertases in chloroplasts, mitochondria, and bacteria. Due to independent gene duplications, all organisms possess two isoforms, Oxa1 and Oxa2 except gram-negative bacteria, which encode only for one YidC-like protein. The genome of Arabidopsis thaliana however, encodes for eight different isoforms. The localization of three of these isoforms has been identified earlier: Alb3 and Alb4 located in thylakoid membranes of chloroplasts while AtOxa1 was found in the inner membrane of mitochondria. Here, we show that the second Oxa1 protein, Oxa1b as well as two Oxa2 proteins are also localized in mitochondria. The last two isoforms most likely encode truncated versions of Oxa-like proteins, which might be inoperable pseudogenes. Homozygous mutant lines were only obtained for Oxa1b, which did not reveal any significant phenotypes, while T-DNA insertion lines of Oxa1a, Oxa2a and Oxa2b resulted only in heterozygous plants indicating that these genes are indispensable for plant development. Phenotyping heterozygous lines showed that embryos are either retarded in growth, display an albino phenotype or embryo formation was entirely abolished suggesting that Oxa1a and both Oxa2 proteins function in embryo formation although at different developmental stages as indicated by the various phenotypes observed.

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Abbreviations

UTR:

Untranslated regions

Met:

Methionine

Val:

Valinomycin

IMS:

Intermembrane space

GFP:

Green fluorescence protein

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Acknowledgments

We thank Prof. Andreas Weber (University Düsseldorf, Germany) for the CFP-PTS1 clone, Dr. Chris Hawes (Bookes University, Osford, UK) for the ST-GFP clone and Dr. Barbara Pickard (Washington University, St. Luis, USA) for the SP + ER-GFP, dcp.r-GFP and mtio-d2r clones. This work was supported by the Deutsche Forschungsgemeinschaft SFB-TR1 project C4.

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Correspondence to Elisabeth Ankele.

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A contribution to the Special Issue on Evolution and Biogenesis of Chloroplasts and Mitochondria.

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Benz, M., Soll, J. & Ankele, E. Arabidopsis thaliana Oxa proteins locate to mitochondria and fulfill essential roles during embryo development. Planta 237, 573–588 (2013). https://doi.org/10.1007/s00425-012-1793-9

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Keywords

  • Alb3/Oxa1/YidC membrane protein family
  • Arabidopsis
  • Embryo lethality
  • Insertase
  • Mitochondria