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CHUP1 mediates actin-based light-induced chloroplast avoidance movement in the moss Physcomitrella patens

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Abstract

Chloroplasts change their intracellular distribution in response to light intensity. CHUP1 (CHLOROPLAST UNUSUAL POSITIONING1) is indispensable for this response in Arabidopsis thaliana. However, involvement of CHUP1 in light-induced chloroplast movement is unknown in other plants. In this study, CHUP1 orthologues were isolated from a moss, Physcomitrella patens, and a fern, Adiantum capillus-veneris, by cDNA library screening and PCR cloning based on the P. patens genome sequence. Functional motifs found in CHUP1 of A. thaliana were conserved among the CHUP1 orthologues. In addition to the putative functional regions, the C-terminal regions (approximately 250 amino acids), which are unique in CHUP1s, were highly conserved. Green fluorescent protein (GFP) fusions of P. patens CHUP1s (PpCHUP1A, PpCHUP1B and PpCHUP1C) were transiently expressed in protoplast cells. All GFP fusions were localized on the chloroplasts. Light-induced chloroplast avoidance movement of chup1 disruptants of P. patens was examined in the presence of cytoskeletal inhibitors because of the utilization of both microtubules and actin filaments for the movement in P. patens. When actin filaments were disrupted by cytochalasin B, the wild type (WT) and all chup1 disruptants showed chloroplast avoidance movement. However, when microtubules were disrupted by Oryzalin, chloroplasts in ∆chup1A and ∆chup1A/B rarely moved and stayed in the strong light-irradiated area. On the other hand, WT, ∆chup1B and ∆chup1C showed chloroplast avoidance movement. These results suggest that PpCHUP1A predominantly mediates the actin-based light-induced chloroplast avoidance movement. This study reveals that CHUP1 functions on the chloroplasts and is involved in the actin-based light-induced chloroplast avoidance movement in P. patens.

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Abbreviations

CHUP1:

Chloroplast unusual positioning1

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Acknowledgments

We thank A. Kadota (Tokyo Metropolitan University) for many helpful suggestions. This work was supported by a Grant-in-Aid for Scientific Research (19770038 to M. K.).

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Authors and Affiliations

  1. Graduate School of Science and Engineering, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu, Shiga, 525-8577, Japan

    Hiroka Usami, Yusuke Fujii & Masahiro Kasahara

  2. Department of Biotechnology, College of Life Sciences, Ritsumeikan University, Nojihigashi, Kusatsu, Shiga, 525-8577, Japan

    Takuma Maeda & Masahiro Kasahara

  3. National Institute for Basic Biology, Okazaki, Aichi, 444-8585, Japan

    Kazusato Oikawa

  4. PRESTO, Japan Science and Technology Agency, Honcho Kawaguchi, Saitama, 332-0012, Japan

    Fumio Takahashi

  5. National Institute of Agrobiological Sciences, Kannondai, Tsukuba, Ibaraki, 305-8602, Japan

    Takatoshi Kagawa

  6. Department of Biology, Faculty of Sciences, Kyushu University, Hakozaki, Fukuoka, 812-8581, Japan

    Masamitsu Wada

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  1. Hiroka Usami
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  2. Takuma Maeda
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  3. Yusuke Fujii
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  4. Kazusato Oikawa
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  6. Takatoshi Kagawa
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  8. Masahiro Kasahara
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Correspondence to Masahiro Kasahara.

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Usami, H., Maeda, T., Fujii, Y. et al. CHUP1 mediates actin-based light-induced chloroplast avoidance movement in the moss Physcomitrella patens . Planta 236, 1889–1897 (2012). https://doi.org/10.1007/s00425-012-1735-6

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  • DOI: https://doi.org/10.1007/s00425-012-1735-6

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