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bZIP transcription factor OsbZIP52/RISBZ5: a potential negative regulator of cold and drought stress response in rice

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Abstract

OsbZIP52/RISBZ5 is a member of the basic leucine zipper (bZIP) transcription factor (TF) family in rice (Oryza sativa) isolated from rice (Zhonghua11) panicles. Expression of the OsbZIP52 gene was strongly induced by low temperature (4°C) but not by drought, PEG, salt, or ABA. The subcellular localization of OsbZIP52-GFP in onion (Allium cepa) epidermis cells revealed that OsbZIP52 is a nuclear localized protein. A transactivation assay in yeast demonstrated that OsbZIP52 functions as a transcriptional activator and can specifically bind to the G-box promoter motif. In a yeast two-hybrid (Y-2-H) experiment, OsbZIP52 was able to form homodimeric complexes. Rice plants overexpressing OsbZIP52 showed significantly increased sensitivity to cold and drought stress. Real-time PCR analysis revealed that some abiotic stress-related genes, such as OsLEA3, OsTPP1, Rab25, gp1 precursor, β-gal, LOC_Os05g11910 and LOC_Os05g39250, were down-regulated in OsbZIP52 overexpression lines. These results suggest that OsbZIP52/RISBZ5 could function as a negative regulator in cold and drought stress environments.

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Abbreviations

bZIP:

Basic leucine zipper

LEA:

Rice late embryogenesis abundant protein

TPP:

Trehalose-6-phosphate phosphatase

gp1:

Glycoprotein 1

RAB:

Responsive to abscisic acid

O2:

Opaque-2

RITA-1:

Rice transcription activator-1

RISBZ:

Rice seed b-Zipper

G/HBF1:

G-box/H-box binding factors

BZO2H:

Basic leucine zipper O2 homolog

Lip19:

Low-temperature-induced protein 19

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Acknowledgments

This research was supported by grants from the Agricultural Ministry of China (2011ZX08009-003-002), and the National Natural Science Foundation of China (2010 No. 31071378).

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Authors and Affiliations

  1. Key Laboratory of Gene Engineering Drug and Biotechnology, Key Laboratory of Cell Proliferation and Regulation of Ministry of Education, College of Life Sciences, Beijing Normal University, Beijing, 100875, People’s Republic of China

    Citao Liu & Xiping Wang

  2. National Center for Molecular Crop Design, Weiming Kaituo Agriculture Biotech Co., Ltd, Beijing, 100085, People’s Republic of China

    Yanbin Wu & Xiping Wang

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  1. Citao Liu
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  2. Yanbin Wu
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Correspondence to Xiping Wang.

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425_2011_1564_MOESM1_ESM.doc

Suppl. Fig. S1 Seedling development of OsbZIP52 overexpression transgenic rice plants under ABA treatment. a–b The seed germination rate and seedling growth of OsbZIP52-overexpression rice lines with and without ABA. c Shoot and root lengths and root number at different ABA concentrations in the WT control (ZH11) and the transgenic rice lines. d Seedlings grown at different concentrations of ABA for 12 days. All the experiments were repeated at least three times (150 seeds for each replicate). ZH11, wild-type Zhonghua11 rice variety. 197-1, -3, -16, -24, -27 are independent transgenic lines of OsbZIP52 (DOC 2055 kb)

425_2011_1564_MOESM2_ESM.doc

Suppl. Fig. S2 OsbZIP52 expression in three rice varieties. a OsbZIP52 expression patterns in different tissues of rice variety Nipponbare. b–d Expression of OsbZIP52 in three rice varieties grown at low temperature (4°C) measured by real-time PCR. DAF, days after flowering (DOC 333 kb)

425_2011_1564_MOESM3_ESM.doc

Suppl. Table S1. Primers used in semi-quantitative RT-PCR and real-time PCR analysis and constructs for vectors (DOC 44 kb)

425_2011_1564_MOESM4_ESM.doc

Suppl. Table S2 Cis-acting elements analysis in promoters of stress-regulated genes in OsbZIP52 overexpression lines. (DOC 31 kb)

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Liu, C., Wu, Y. & Wang, X. bZIP transcription factor OsbZIP52/RISBZ5: a potential negative regulator of cold and drought stress response in rice. Planta 235, 1157–1169 (2012). https://doi.org/10.1007/s00425-011-1564-z

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