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Planta

, Volume 235, Issue 3, pp 565–578 | Cite as

Functional characterization of an acidic SK3 dehydrin isolated from an Opuntia streptacantha cDNA library

  • A. E. Ochoa-Alfaro
  • M. Rodríguez-Kessler
  • M. B. Pérez-Morales
  • P. Delgado-Sánchez
  • C. L. Cuevas-Velazquez
  • G. Gómez-Anduro
  • J. F. Jiménez-Bremont
Original Article

Abstract

Cactus pears are succulent plants of the Cactaceae family adapted to extremely arid, hot and cold environments, making them excellent models for the study of molecular mechanisms underlying abiotic stress tolerance. Herein, we report a directional cDNA library from 12-month-old cladodes of Opuntia streptacantha plants subjected to abiotic stresses. A total of 442 clones were sequenced, representing 329 cactus pear unigenes, classified into eleven functional categories. The most abundant EST (unigen 33) was characterized under abiotic stress. This cDNA of 905 bp encodes a SK3-type acidic dehydrin of 248 amino acids. The OpsDHN1 gene contains an intron inserted within the sequence encoding the S-motif. qRT-PCR analysis shows that the OpsDHN1 transcript is specifically accumulated in response to cold stress, and induced by abscisic acid. Over-expression of the OpsDHN1 gene in Arabidopsis thaliana leads to enhanced tolerance to freezing treatment, suggesting that OpsDHN1 participates in freezing stress responsiveness. Generation of the first EST collection for the characterization of cactus pear genes constitutes a useful platform for the understanding of molecular mechanisms of stress tolerance in Opuntia and other CAM plants.

Keywords

Abiotic stress Cactus pear cDNA library Cold stress Dehydrin 

Abbreviations

ABA

Abscisic acid

CAM

Crassulacean acid metabolism

ORF

Open reading frame

OpsDHN1

Opuntia streptacantha dehydrin 1

qRT-PCR

Quantitative reverse transcriptase-polymerase chain reaction

Notes

Acknowledgments

This work was supported by SAGARPA (2004-C01-216) and the CONACYT (Investigación Ciencia Básica 2008-103106) fundings. We are grateful to Dr. Paul Riley for a grammatical review.

Supplementary material

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • A. E. Ochoa-Alfaro
    • 1
  • M. Rodríguez-Kessler
    • 2
  • M. B. Pérez-Morales
    • 1
  • P. Delgado-Sánchez
    • 1
  • C. L. Cuevas-Velazquez
    • 1
  • G. Gómez-Anduro
    • 3
  • J. F. Jiménez-Bremont
    • 1
  1. 1.Division de Biologia MolecularInstituto Potosino de Investigacion Cientifica y Tecnologica, Camino a la Presa de San Jose 2055San Luis PotosiMexico
  2. 2.Facultad de CienciasUniversidad Autonoma de San Luis PotosiSan Luis PotosiMexico
  3. 3.Agricultura en Zonas Aridas, Centro de Investigaciones Biologicas del Noreste Mar Bermejo No. 195, Col. Playa Palo de Santa RitaLa PazMexico

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