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Planta

, Volume 235, Issue 3, pp 523–537 | Cite as

Overexpression of Nelumbo nucifera metallothioneins 2a and 3 enhances seed germination vigor in Arabidopsis

  • Yuliang Zhou
  • Pu Chu
  • Huhui Chen
  • Yin Li
  • Jun Liu
  • Yu Ding
  • Edward W. T. Tsang
  • Liwen Jiang
  • Keqiang Wu
  • Shangzhi Huang
Original Article

Abstract

Metallothioneins (MTs) are small, cysteine-rich and metal-binding proteins which are involved in metal homeostasis and scavenging of reactive oxygen species. Although plant MTs have been intensively studied, their roles in seeds remain to be clearly established. Here, we report the isolation and characterization of NnMT2a, NnMT2b and NnMT3 from sacred lotus (Nelumbo nucifera Gaertn.) and their roles in seed germination vigor. The transcripts of NnMT2a, NnMT2b and NnMT3 were highly expressed in developing and germinating sacred lotus seeds, and were dramatically up-regulated in response to high salinity, oxidative stresses and heavy metals. Analysis of transformed Arabidopsis protoplasts showed that NnMT2a-YFP and NnMT3-YFP were localized in cytoplasm and nucleoplasm. Transgenic Arabidopsis seeds overexpressing NnMT2a and NnMT3 displayed improved resistance to accelerated aging (AA) treatment, indicating their significant roles in seed germination vigor. These transgenic seeds also exhibited higher superoxide dismutase activity compared to wild-type seeds after AA treatment. In addition, we showed that NnMT2a and NnMT3 conferred improved germination ability to NaCl and methyl viologen on transgenic Arabidopsis seeds. Taken together, these data demonstrate that overexpression of NnMT2a and NnMT3 in Arabidopsis significantly enhances seed germination vigor after AA treatment and under abiotic stresses.

Keywords

Arabidopsis Metallothionein Oxidative stresses Sacred lotus Salt stress Seed germination vigor 

Abbreviations

AA

Accelerated aging

BAR

Bio-array resource for plant biology

Cys

Cysteine

DAP

Days after pollination

ESTs

Expressed sequence tags

MTs

Metallothioneins

MV

Methyl viologen

NBT

Nitro blue tetrazolium

SOD

Superoxide dismutase

YFP

Yellow fluorescent protein

Notes

Acknowledgments

This study was supported by Natural Science Foundation of China (30370912), Natural Science Foundation of Guangdong Province (2006B20101010 and 9151027501000075) and Guangdong Agriculture Science and Technology Team Project to S. Huang and Guangdong Provincial Science and Technology Program (2010D020301003) to J Liu.

Supplementary material

425_2011_1527_MOESM1_ESM.pdf (645 kb)
Online Resources 1–7 (PDF 644 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Yuliang Zhou
    • 1
  • Pu Chu
    • 1
  • Huhui Chen
    • 1
  • Yin Li
    • 1
  • Jun Liu
    • 2
  • Yu Ding
    • 3
  • Edward W. T. Tsang
    • 4
  • Liwen Jiang
    • 3
  • Keqiang Wu
    • 5
  • Shangzhi Huang
    • 1
  1. 1.Guangdong Provincial Key Laboratory of Plant Resource, School of Life SciencesSun Yat-Sen UniversityGuangzhouChina
  2. 2.Guangdong Academy of Agricultural SciencesGuangzhouChina
  3. 3.Centre for Cell and Developmental Biology, School of Life SciencesThe Chinese University of Hong KongHong KongChina
  4. 4.Plant Biotechnology InstituteNational Research Council of CanadaSaskatoonCanada
  5. 5.Institute of Plant Biology, College of Life ScienceNational Taiwan UniversityTaipei 106Taiwan

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