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Increased tolerance to salt stress in the phosphate-accumulating Arabidopsis mutants siz1 and pho2

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Abstract

High salinity is an environmental factor that inhibits plant growth and development, leading to large losses in crop yields. We report here that mutations in SIZ1 or PHO2, which cause more accumulation of phosphate compared with the wild type, enhance tolerance to salt stress. The siz1 and pho2 mutations reduce the uptake and accumulation of Na+. These mutations are also able to suppress the Na+ hypersensitivity of the sos3-1 mutant, and genetic analyses suggest that SIZ1 and SOS3 or PHO2 and SOS3 have an additive effect on the response to salt stress. Furthermore, the siz1 mutation cannot suppress the Li+ hypersensitivity of the sos3-1 mutant. These results indicate that the phosphate-accumulating mutants siz1 and pho2 reduce the uptake and accumulation of Na+, leading to enhanced salt tolerance, and that, genetically, SIZ1 and PHO2 are likely independent of SOS3-dependent salt signaling.

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Abbreviations

ICP-AES:

Inductively coupled plasma-atomic emission spectrometry

MS:

Murashige and Skoog

NaPi:

Na+-coupled phosphate transporter

Pi:

Phosphate

ROS:

Reactive oxygen species

SA:

Salicylic acid

SUMO:

Small ubiquitin-related modifier

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Acknowledgments

We thank Ms. Miyuki Hara, Ms. Risa Osada, Ms. Ami Komata, and Ms. Ayaka Sato for technical support. We are grateful to the Chemical Analysis Center, University of Tsukuba, for use of the ICP-AES spectrometer. This work was supported, in part, by a Special Coordination Funds for Promoting Science and Technology grant from the Ministry of Education, Culture, Sports, Science, and Technology, the Japanese Government, and by The Salt Science Research Foundation, No. 1023.

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  1. Masaru Ohta

    Present address: Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences, Tsukuba, 305-8602, Japan

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  1. Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 305-8572, Japan

    Kenji Miura, Aiko Sato, Masaru Ohta & Jun Furukawa

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  1. Kenji Miura
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  2. Aiko Sato
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Correspondence to Kenji Miura.

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The contributions of K. Miura and A. Sato are considered to be equal.

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Miura, K., Sato, A., Ohta, M. et al. Increased tolerance to salt stress in the phosphate-accumulating Arabidopsis mutants siz1 and pho2 . Planta 234, 1191–1199 (2011). https://doi.org/10.1007/s00425-011-1476-y

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