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Up-regulation of leucine aminopeptidase-A in cadmium-treated tomato roots

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Abstract

The effects of cadmium (Cd) on aminopeptidase (AP) activities and Leucine-AP (LAP) expression were investigated in the roots of tomato (Solanum lycopersicum L., var Ibiza) plants. Three-week-old plants were grown for 10 days in the presence of 0.3–300 μM Cd and compared to control plants grown in the absence of Cd. AP activities were measured using six different p-nitroanilide (p-NA) substrates. Leu, Met, Arg, Pro and Lys hydrolyzing activities increased in roots of Cd-treated plants, while Phe-pNA cleavage was not enhanced after Cd treatments. The use of peptidase inhibitors showed that most of the Leu-pNA hydrolyzing activity was related to one or several metallo-APs. Changes in Lap transcripts, protein and activities were measured in the roots of 0 and 30-μM Cd-treated plants. LapA transcript levels increased in Cd-treated roots, whereas LapN RNAs levels were not modified. To assess amount of Leu-pNA hydrolyzing activity associated with the hexameric LAPs, LAP activity was measured following immunoprecipitation with a LAP polyclonal antiserum. LAP activity increased in Cd-treated roots. There was a corresponding increase in LAP-A protein levels detected in 2D-immunoblots. The role of LAP-A in the proteolytic response to Cd stress is discussed.

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Fig. 1
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Abbreviations

AP:

Aminopeptidase

Cd:

Cadmium

LAP:

Leucine aminopeptidase

PMSF:

Phenylmethylsulfonyl fluoride

pNA:

p-Nitroanilide

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Authors and Affiliations

  1. Département de Biologie, Faculté des Sciences de Tunis El Manar, Unité de Recherche de Biologie et Physiologie Cellulaires Végétales, 1060, Tunis, Tunisia

    Latifa Boulila-Zoghlami, Whabi Djebali & Wided Chaïbi

  2. UMR de Biologie du Fruit et Pathologie, INRA-Universités de Bordeaux 1 et Bordeaux 2, BP 81, 33883, Villenave d’Ornon, France

    Philippe Gallusci

  3. Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, CA, 95521-0124, USA

    Frances M. Holzer & Linda L. Walling

  4. Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA

    Gilles J. Basset

  5. UMR INRA 1301, CNRS 6243, Université de Nice Sophia Antipolis, Interactions Biotiques & Santé Végétale, Institut Sophia Agrobiotech, 400 Route des Chappes, BP 167, 06903, Sophia Antipolis Cedex, France

    Renaud Brouquisse

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  1. Latifa Boulila-Zoghlami
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  2. Philippe Gallusci
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  3. Frances M. Holzer
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  4. Gilles J. Basset
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  5. Whabi Djebali
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  6. Wided Chaïbi
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  7. Linda L. Walling
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  8. Renaud Brouquisse
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Correspondence to Renaud Brouquisse.

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Boulila-Zoghlami, L., Gallusci, P., Holzer, F.M. et al. Up-regulation of leucine aminopeptidase-A in cadmium-treated tomato roots. Planta 234, 857–863 (2011). https://doi.org/10.1007/s00425-011-1468-y

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  • DOI: https://doi.org/10.1007/s00425-011-1468-y

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