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A role for the anaphase promoting complex in hormone regulation

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Abstract

To increase our knowledge of anaphase promoting complex (APC/C) function during plant development, we characterized an Arabidopsis thaliana T-DNA-insertion line where the T-DNA fell within the 5′ regulatory region of the APC10 gene. The insert disrupted endogenous expression, resulting in overexpression of APC10 mRNA from the T-DNA- internal CaMV 35S promoter, and increased APC10 protein. Overexpression of APC10 produced phenotypes resembling those of known auxin and ethylene mutants, and increased expression of two tested auxin-regulated genes, small auxin up RNA (SAUR) 15 and SAUR24. Taken together, our data suggests that elevated APC10 likely mimics auxin and ethylene sensitive phenotypes, expanding our understanding of proteolytic processes in hormone regulation of plant development.

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Abbreviations

APC/C:

Anaphase promoting complex/cyclosome

apc10 OE:

APC10 overexpressor

CHS:

Chalcone synthase

EIN3:

Ethylene-insensitive3

ERF1 :

Ethylene response factor1

erh3 :

Ectopic root hair3 mutant

NAA:

α-Naphthaleneacetic acid

SAUR :

Small auxin up RNA

SCF:

Skp1-Cullin-F-box

tt4/chs :

Transparent testa4/chalcone synthase mutant

WT:

Wild-type

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Acknowledgments

We are grateful to Lindsay Yaworsky for initial observations, Rory Degenhardt, Sarah Caldwell and Joyce Wilson for technical assistance. This work was supported by Natural Sciences and Engineering Research Council (NSERC) of Canada Discovery grants to P.C.B–S. and G.R.G., Canadian Foundation for Innovation (CFI) to T.A.A.H. and Canadian Institutes for Health Research (CIHR)/Saskatchewan Health Research Foundation (SHRF) to D.L.L.

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Authors and Affiliations

  1. Department of Anatomy and Cell Biology, University of Saskatchewan, Saskatoon, SK, S7N 5E5, Canada

    Donna L. Lindsay, Spike Postnikoff & Troy A. A. Harkness

  2. Department of Biology, University of Saskatchewan, Saskatoon, SK, S7N 5E2, Canada

    Peta C. Bonham-Smith

  3. Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK, S7N 5A8, Canada

    Gordon R. Gray

  4. Department of Biochemistry, University of Saskatchewan, Saskatoon, SK, S7N 5E5, Canada

    Gordon R. Gray

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  1. Donna L. Lindsay
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  5. Troy A. A. Harkness
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Correspondence to Troy A. A. Harkness.

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Lindsay, D.L., Bonham-Smith, P.C., Postnikoff, S. et al. A role for the anaphase promoting complex in hormone regulation. Planta 233, 1223–1235 (2011). https://doi.org/10.1007/s00425-011-1374-3

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