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Leaf proteomic analysis in cassava (Manihot esculenta, Crantz) during plant development, from planting of stem cutting to storage root formation

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Abstract

Tuberization in cassava (Manihot esculenta Crantz) occurs simultaneously with plant development, suggesting competition of photoassimilate partitioning between the shoot and the root organs. In potato, which is the most widely studied tuber crop, there is ample evidence suggesting that metabolism and regulatory processes in leaf may have an impact on tuber formation. To search for leaf proteins putatively involved in regulating tuber generation and/or development in cassava, comparative proteomic approaches have been applied to monitor differentially expressed leaf proteins during root transition from fibrous to tuberous. Stringent cross comparison and statistical analysis between two groups with different plant ages using Student’s t test with 95% significance level revealed a number of protein spots whose abundance were significantly altered (P < 0.05) during week 4 to week 8 of growth. Of these, 39 spots were successfully identified by ion trap LC–MS/MS. The proteins span various functional categories from antioxidant and defense, carbohydrate metabolism, cyanogenesis, energy metabolism, miscellaneous and unknown proteins. Results suggested possible metabolic switches in the leaf that may trigger/regulate storage root initiation and growth. This study provides a basis for further functional characterization of differentially expressed leaf proteins, which can help understand how biochemical processes in cassava leaves may be involved in storage root development.

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Abbreviations

2-DE:

2-Dimensional gel electrophoresis

CBB:

Coomassie brilliant blue

LC–MS/MS:

Liquid chromatography coupled with tandem mass spectrometry

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Acknowledgments

This work was financially supported in part by Mahidol University and National Center for Genetic Engineering and Biotechnology (BIOTEC) grant # BT-B-01-PM-14-4806.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, Center for Cassava Molecular Biotechnology, Center for Excellence in Protein Structure and Function, Mahidol University, 272 Rama 6 Rd., Bangkok, 10400, Thailand

    Mashamon Mitprasat & Kittisak Yokthongwattana

  2. Genome Institute, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Phahonyothin Rd., Pathumthani, 12120, Thailand

    Sittiruk Roytrakul & Surasak Jiemsup

  3. Department of Agriculture, Rayong Field Crop Research Center, Ministry of Agriculture and Cooperatives, Rayong, 21150, Thailand

    Opas Boonseng

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  1. Mashamon Mitprasat
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  2. Sittiruk Roytrakul
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  3. Surasak Jiemsup
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  4. Opas Boonseng
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  5. Kittisak Yokthongwattana
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Correspondence to Kittisak Yokthongwattana.

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Mitprasat, M., Roytrakul, S., Jiemsup, S. et al. Leaf proteomic analysis in cassava (Manihot esculenta, Crantz) during plant development, from planting of stem cutting to storage root formation. Planta 233, 1209–1221 (2011). https://doi.org/10.1007/s00425-011-1373-4

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