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AtAGP18 is localized at the plasma membrane and functions in plant growth and development

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Abstract

Arabinogalactan-proteins (AGPs) are a family of highly glycosylated hydroxyproline-rich glycoproteins (HRGPs). AtAGP17, 18 and 19 comprise the lysine-rich classical AGP subfamily in Arabidopsis. Overexpression of GFP–AtAGP17/18/19 fusion proteins in Arabidopsis revealed localization of the fusion proteins on the plant cell surface of different organs. Subcellular localization of the fusion proteins at the plasma membrane was further determined by plasmolysis of leaf trichome cells. To elucidate AtAGP17/18/19 function(s), these AGPs were expressed without the green fluorescent protein (GFP) tag under the control of 35S cauliflower mosaic virus promoter. In contrast to AtAGP17/AtAGP19 overexpressors which showed phenotypes identical to wild-type plants, AtAGP18 overexpressors displayed several phenotypes distinct from wild-type plants. Specifically, these overexpressors had smaller rosettes and shorter stems and roots, produced more branches and had less viable seeds. Moreover, these AtAGP18 overexpressors exhibited similar phenotypes to tomato LeAGP-1 overexpressors, suggesting these two AGP genes may have similar function(s) in Arabidopsis and tomato.

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Abbreviations

ABA:

Abscisic acid

AGP:

Arabinogalactan-protein

CaMV:

Cauliflower mosaic virus

GFP:

Green fluorescent protein

GPI:

Glycosylphosphatidylinositol

GUS:

β-Glucuronidase

MS:

Murashige and Skoog

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Acknowledgments

We are particularly grateful to Dr. Li Tan in the Department of Chemistry and Biochemistry at Ohio University for providing the pUC-ss-GFP construct. This work was supported by the National Science Foundation [IBN-0110413].

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Authors and Affiliations

  1. Department of Environmental and Plant Biology, Molecular and Cellular Biology Program, Ohio University, 504 Porter Hall, Athens, OH, 45701-2979, USA

    Yizhu Zhang & Allan M. Showalter

  2. College of Biological Science and Technology, Fuzhou University, Fuzhou, 350108, China

    Jie Yang

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  1. Yizhu Zhang
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  2. Jie Yang
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  3. Allan M. Showalter
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Correspondence to Allan M. Showalter.

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Zhang, Y., Yang, J. & Showalter, A.M. AtAGP18 is localized at the plasma membrane and functions in plant growth and development. Planta 233, 675–683 (2011). https://doi.org/10.1007/s00425-010-1331-6

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