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Planta

, Volume 233, Issue 3, pp 593–609 | Cite as

StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling

  • Verónica Giammaria
  • Carolina Grandellis
  • Sandra Bachmann
  • Pablo Rubén Gargantini
  • Sergio Enrique Feingold
  • Glenn Bryan
  • Rita María Ulloa
Original Article

Abstract

Calcium-dependent protein kinases (CDPKs) are essential calcium sensors. In this work, we have studied StCDPK2 isoform from potato both at gene and protein level. StCdpk2 genomic sequence contains eight exons and seven introns, as was observed for StCdpk1. There is one copy of the gene per genome located in chromosome 7. StCDPK2 encodes an active CDPK of 515 aminoacids, with an apparent MW of 57 kDa, which presents myristoylation and palmitoylation consensus in its N-terminus. StCDPK2 is highly expressed in leaves and green sprouts; enhanced expression was detected under light treatment, which corresponds well with light responsive cis-acting elements found in its promoter sequence. Antibodies against the recombinant StCDPK2::6xHis protein detected this isoform in soluble and particulate fractions from leaves. StCDPK2 autophosphorylation and kinase activity are both calcium dependent reaching half maximal activation at 0.6 μM calcium. The active kinase is autophosphorylated on serine and tyrosine residues and its activity is negatively modulated by phosphatidic acid (PA). Our results reveal StCDPK2 as a signalling element involved in plant growth and development and show that its activity is tightly regulated.

Keywords

Autophosphorylation CDPK Light Phospholipids Solanum tuberosum 

Abbreviations

ABA

Absicic acid

CDPK

Calcium-dependent protein kinase

DGPP

Diacylglycerol pyrophosphate

JA

Jasmonic acid

PA

Phosphatidic acid

Notes

Acknowledgments

We thank technical assistance of Karen McClean. We thank Dr. Sara Maldonado for helpful discussion and the technical assistance of Verónica Lainez. We thank Dr Salomé Prat Monguío (CSIC, Madrid, Spain) for the cDNA library from tuberizing stolons and Dr. Ana L. Villasuso and Dr. Estela E. Machado for providing the phospholipids. This work was supported by grants from CONICET, UBA, FONCYT-ANPCYT and the Royal Society of Edinburgh. RMU and PRG are members of CONICET. VG, CG and SB are fellows from CONICET.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Verónica Giammaria
    • 1
  • Carolina Grandellis
    • 1
  • Sandra Bachmann
    • 1
  • Pablo Rubén Gargantini
    • 1
    • 4
  • Sergio Enrique Feingold
    • 2
  • Glenn Bryan
    • 3
  • Rita María Ulloa
    • 1
  1. 1.Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI), CONICET and Facultad de Ciencias Exactas y NaturalesUniversidad de Buenos AiresBuenos AiresArgentina
  2. 2.Laboratorio de AgrobiotecnologíaEEA Balcarce-INTABalcarceArgentina
  3. 3.Genetics ProgrammeScottish Crop Research Institute (SCRI)DundeeUK
  4. 4.Facultad de MedicinaUniversidad Católica de Córdoba (UCC)CórdobaArgentina

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