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Membrane fusion process and assembly of cell wall during cytokinesis in the brown alga, Silvetia babingtonii (Fucales, Phaeophyceae)

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Abstract

During cytokinesis in brown algal cells, Golgi-derived vesicles (GVs) and flat cisternae (FCs) are involved in building the new cell partition membrane. In this study, we followed the membrane fusion process in Silvetia babingtonii zygotes using electron microscopy together with rapid freezing and freeze substitution. After mitosis, many FCs were formed around endoplasmic reticulum clusters and these then spread toward the future cytokinetic plane. Actin depolymerization using latrunculin B prevented the appearance of the FCs. Fusion of GVs to FCs resulted in structures that were thicker and more elongated (EFCs; expanded flat cisternae). Some complicated membranous structures (MN; membranous network) were formed by interconnection of EFCs and following the arrival of additional GVs. The MN grew into membranous sacs (MSs) as gaps between the MNs disappeared. The MSs were observed in patches along the cytokinetic plane. Neighboring MSs were united to form the new cell partition membrane. An immunocytochemical analysis indicated that fucoidan was synthesized in Golgi bodies and transported by vesicles to the future cytokinetic plane, where the vesicles fused with the FCs. Alginate was not detected until the MS phase. Incubation of sections with cellulase-gold showed that the cellulose content of the new cross wall was not comparable to that of the parent cell wall.

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Abbreviations

EFC:

Expanded flat cisterna

ER:

Endoplasmic reticulum

FC:

Flat cisterna

GV:

Golgi-derived vesicle

LB:

Latrunculin B

MN:

Membranous network

MS:

Membranous sac

MT:

Microtubule

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Acknowledgments

We thank Dr. C. Katsaros, University of Athens, Faculty of Biology, Department of Botany and Dr. J.M. Cock, Station Biologique de Roscoff for their critical reading of the manuscript and helpful suggestions. This study was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan (21770065, 20370025), and by the Research and Development Program for New Bio-industry Initiatives (2006-2010) of Bio-oriented Technology Research Advancement Institution (BRAIN), Japan.

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Authors and Affiliations

  1. Muroran Marine Station, Field Science Center for Northern Biosphere, Hokkaido University, Muroran, 051-0003, Japan

    Chikako Nagasato & Taizo Motomura

  2. Graduate School of Fisheries Science, Hokkaido University, Hakodate, 041-8611, Japan

    Akira Inoue & Takao Ojima

  3. Graduate School of Agricultural Science, Kobe University, Kobe, 657-8501, Japan

    Masashi Mizuno & Kazuki Kanazawa

  4. Graduate School of Kuroshio Science, Kochi University, 2-5-1 Akebono-cho, Kochi, 780-8520, Japan

    Kazuo Okuda

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  1. Chikako Nagasato
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  2. Akira Inoue
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  3. Masashi Mizuno
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  4. Kazuki Kanazawa
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  5. Takao Ojima
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  6. Kazuo Okuda
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  7. Taizo Motomura
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Correspondence to Chikako Nagasato.

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Nagasato, C., Inoue, A., Mizuno, M. et al. Membrane fusion process and assembly of cell wall during cytokinesis in the brown alga, Silvetia babingtonii (Fucales, Phaeophyceae). Planta 232, 287–298 (2010). https://doi.org/10.1007/s00425-010-1188-8

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