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Effects of MULTIFOLIATE-PINNA, AFILA, TENDRIL-LESS and UNIFOLIATA genes on leafblade architecture in Pisum sativum

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Abstract

In order to dissect the genetic regulation of leafblade morphogenesis, 16 genotypes of pea, constructed by combining the wild-type and mutant alleles of MFP, AF, TL and UNI genes, were quantitatively phenotyped. The morphological features of the three domains of leafblades of four genotypes, unknown earlier, were described. All the genotypes were found to differ in leafblade morphology. It was evident that MFP and TL functions acted as repressor of pinna ramification, in the distal domain. These functions, with and without interaction with UNI, also repressed the ramification of proximal pinnae in the absence of AF function. The expression of MFP and TL required UNI function. AF function was found to control leafblade architecture multifariously. The earlier identified role of AF as a repressor of UNI in the proximal domain was confirmed. Negative control of AF on the UNI-dependent pinna ramification in the distal domain was revealed. It was found that AF establishes a boundary between proximal and distal domains and activates formation of leaflet pinnae in the proximal domain.

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Abbreviations

AF, af:

AFILA

AS1:

ASYMMETRIC LEAVES1

CRI, cri:

CRISPA

FLO:

FLORICAULA

LFY:

LEAFY

MFP, mfp:

MULTIFOLIATE-PINNA

STP, stp:

STAMINA-PISTILLOIDA

TL, tl:

TENDRIL-LESS

UNI, uni:

UNIFOLIATA

uni-tac:

Unifoliata-tendrilled acacia

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Acknowledgments

Grateful thanks are due to the Director, NIPGR for providing facilities, Indian National Science Academy for sanctioning Senior Scientistship Scheme to SK and to Mukesh Jain for reading the manuscript. The help of Vinod Kumar and Sunil Kumar in field work is also thankfully acknowledged.

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Authors and Affiliations

  1. National Institute for Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, Post Box 10531, New Delhi, 110067, India

    Raghvendra Kumar Mishra, Swati Chaudhary & Sushil Kumar

  2. School of Biotechnology, Devi Ahilya University, Indore, 452001, India

    Raghvendra Kumar Mishra & Anil Kumar

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  1. Raghvendra Kumar Mishra
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  2. Swati Chaudhary
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  4. Sushil Kumar
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Correspondence to Sushil Kumar.

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Mishra, R.K., Chaudhary, S., Kumar, A. et al. Effects of MULTIFOLIATE-PINNA, AFILA, TENDRIL-LESS and UNIFOLIATA genes on leafblade architecture in Pisum sativum . Planta 230, 177–190 (2009). https://doi.org/10.1007/s00425-009-0931-5

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