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Functional roles of the pepper antimicrobial protein gene, CaAMP1, in abscisic acid signaling, and salt and drought tolerance in Arabidopsis

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Abstract

Biotic signaling molecules including abscisic acid (ABA) are involved in signal transduction pathways that mediate the defense response of plants to environmental stresses. The antimicrobial protein gene CaAMP1, previously isolated from pepper (Capsicum annuum), was strongly induced in pepper leaves exposed to ABA, NaCl, drought, or low temperature. Because transformation is very difficult in pepper, we overexpressed CaAMP1 in Arabidopsis. CaAMP1-overexpressing (OX) transgenic plants exhibited reduced sensitivity to ABA during the seed germination and seedling stages. Overexpression of CaAMP1 conferred enhanced tolerance to high salinity and drought, accompanied by altered expression of the AtRD29A gene, which is correlated with ABA levels and environmental stresses. The transgenic plants were also highly tolerant to osmotic stress caused by high concentrations of mannitol. Together, these results suggest that overexpression of the CaAMP1 transgene modulates salt and drought tolerance in Arabidopsis through ABA-mediated cell signaling.

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Acknowledgments

This work was supported by a grant (CG1133) from the Crop Functional Genomics Center of the 21st Century Frontier Research Program, funded by the Ministry of Education and Science and Technology, Republic of Korea.

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  1. Sung Chul Lee

    Present address: Department of Plant and Microbial Biology, University of California, Berkeley, CA, 94720, USA

Authors and Affiliations

  1. Laboratory of Molecular Plant Pathology, School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-ku, Seoul, 136-713, Republic of Korea

    Sung Chul Lee & Byung Kook Hwang

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  1. Sung Chul Lee
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Correspondence to Byung Kook Hwang.

Additional information

The nucleotide sequence data reported here have been deposited in the GenBank database under the accession number AY548741.

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Lee, S.C., Hwang, B.K. Functional roles of the pepper antimicrobial protein gene, CaAMP1, in abscisic acid signaling, and salt and drought tolerance in Arabidopsis . Planta 229, 383–391 (2009). https://doi.org/10.1007/s00425-008-0837-7

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