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Mycosporine-like amino acids (MAAs) profile of a rice-field cyanobacterium Anabaena doliolum as influenced by PAR and UVR

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Abstract

The mycosporine-like amino acid (MAA) profile of a rice-field cyanobacterium, Anabaena doliolum, was studied under PAR and PAR + UVR conditions. The high-performance liquid chromatographic analysis of water-soluble compounds reveals the biosynthesis of three MAAs, mycosporine-glycine (λ max = 310 nm), porphyra-334 (λ max = 334 nm) and shinorine (λ max = 334 nm), with retention times of 4.1, 3.5 and 2.3 min, respectively. This is the first report for the occurrence of mycosporine-glycine and porphyra-334 in addition to shinorine in Anabaena strains studied so far. The results indicate that mycosporine-glycine (monosubstituted) acts as a precursor for the biosynthesis of the bisubstituted MAAs shinorine and porphyra-334. Mycosporine-glycine was under constitutive control while porphyra-334 and shinorine were induced by UV-B radiation, indicating the involvement of UV-regulated enzymes in the biotransformation of MAAs. It seems that A. doliolum is able to protect its cell machinery from UVR by synthesizing a complex set of MAAs and thus is able to survive successfully during the summer in its natural brightly lit habitats.

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Abbreviations

HPLC:

High-performance liquid chromatography

MAAs:

Mycosporine-like amino acids

PAR:

Photosynthetically active radiation

UVR:

Ultraviolet radiation

UV-A:

Ultraviolet-A

UV-B:

Ultraviolet-B

References

  • Böhm GA, Pfleiderer W, Böger P, Scherer S (1995) Structure of a novel oligosaccharide-mycosporine-amino acid ultraviolet A/B sunscreen pigment from the terrestrial cyanobacterium Nostoc commune. J Biol Chem 270:8536–8539

    Article  PubMed  Google Scholar 

  • Bothwell ML, Sherbot DMJ, Pollock CM (1994) Ecosystem response to solar ultraviolet-B radiation: influence of trophic-level interactions. Science 265:97–100

    Article  PubMed  CAS  Google Scholar 

  • Britt AB (1995) Repair of DNA damage induced by ultraviolet radiation. Plant Physiol 108:891–896

    Article  PubMed  CAS  Google Scholar 

  • Brocks JJ, Logan GA, Buick R, Summons RE (1999) Archean molecular fossils and the early rise of Eukaryotes. Science 285:1033–1036

    Article  PubMed  CAS  Google Scholar 

  • Brown AM (2005) A new software for carrying out one-way ANOVA post hoc tests. Comput Methods Programs Biomed 79:89–95

    Article  PubMed  Google Scholar 

  • Callone AI, Carignan M, Montoya NG, Carreto JI (2006) Biotransformation of mycosporine like amino acids (MAAs) in the toxic dinoflagellate Alexandrium tamarense. J Photochem Photobiol B Biol 84:204–212

    Article  CAS  Google Scholar 

  • Carreto JI, Carignan MO, Daleo G, De Marco SG (1990) Occurrence of mycosporine-like amino acids in the red-tide dinoflagellate Alexandrium excavatum: UV-protective compounds? J Plankton Res 12:909–921

    Article  CAS  Google Scholar 

  • Conde FR, Churio MS, Previtali CM (2004) The deactivation pathways of the excited-states of the mycosporine-like amino acids shinorine and porphyra-334 in aqueous solution. Photochem Photobiol Sci 3:960–967

    Article  PubMed  CAS  Google Scholar 

  • Crutzen PJ (1992) Ultraviolet on the increase. Nature 356:104–105

    Article  Google Scholar 

  • Desikachary TV (1959) Cyanophyta. Indian Council of Agriculture Research, New Delhi, p 410

    Google Scholar 

  • Ehling-Schulz M, Bilger W, Scherer S (1997) UV-B-induced synthesis of photoprotective pigments and extracellular polysaccharides in the terrestrial cyanobacterium Nostoc commune. J Bacteriol 179:1940–1945

    PubMed  CAS  Google Scholar 

  • Franklin LA, Forster RM (1997) The changing irradiance environment: consequences for marine macrophyte physiology, productivity and ecology. Eur J Phycol 32:207–232

    Google Scholar 

  • Gao K, Wu Y, Li G, Wu H, Villafañe VE, Helbling EW (2007a) Solar UV radiation drives CO2 fixation in marine phytoplankton: a double-edged sword. Plant Physiol 144:54–59

    Article  PubMed  CAS  Google Scholar 

  • Gao K, Yu H, Brown MT (2007b) Solar PAR and UV radiation affects the physiology and morphology of the cyanobacterium Anabaena sp. PCC 7120. J Photochem Photobiol B Biol 89:117–124

    Article  CAS  Google Scholar 

  • Garcia-Pichel F, Castenholz RW (1993) Occurrence of UV-absorbing, mycosporine-like compounds among cyanobacterial isolates and an estimate of their screening capacity. Appl Environ Microbiol 59:163–169

    PubMed  CAS  Google Scholar 

  • Garcia-Pichel F, Wingard CE, Castenholz RW (1993) Evidence regarding the UV sunscreen role of a mycosporine-like compound in the cyanobacterium Gloeocapsa sp. Appl Environ Microbiol 59:170–176

    PubMed  CAS  Google Scholar 

  • Häder D-P, Kumar HD, Smith RC, Worrest RC (2007) Effects of solar UV radiation on aquatic ecosystems and interactions with climate change. Photochem Photobiol Sci 6:267–285

    Article  PubMed  CAS  Google Scholar 

  • Han T, Kong J-A, Han Y-S, Kang S-H, Häder D-P (2004) UV-A/blue light-induced reactivation of spore germination in UV-B irradiated Ulva pertusa (Chlorophyta). J Phycol 40:315–322

    Article  Google Scholar 

  • Hedges SB, Chen H, Kumar S, Wang DYC, Thompson AS, Watanabe H (2001) A genomic timescale for the origin of eukaryotes. BMC Evol Biol 1:4

    Article  PubMed  CAS  Google Scholar 

  • Karentz D, Cleaver JE, Mitchell DL (1991) DNA damage in the Antarctic. Nature 350:28

    Article  Google Scholar 

  • Kim S-T, Sancar A (1995) Photorepair of nonadjacent pyrimidine dimers by DNA photolyase. Photochem Photobiol 61:171–174

    Article  PubMed  CAS  Google Scholar 

  • Klisch M, Sinha RP, Richter PR, Häder D-P (2001) Mycosporine-like amino acids (MAAs) protect against UV-B-induced damage in Gyrodinium dorsum Kofoid. J Plant Physiol 158:1449–1454

    Article  CAS  Google Scholar 

  • Lesser MP (2008) Effects of ultraviolet radiation on productivity and nitrogen fixation in the cyanobacterium, Anabaena sp. (Newton’s strain). Hydrobiologia 598:1–9

    Article  CAS  Google Scholar 

  • Lesser MP, Cullen JJ, Neale PJ (1994) Carbon uptake in a marine diatom during acute exposure to ultraviolet B radiation: relative importance of damage and repair. J Phycol 30:183–192

    Article  CAS  Google Scholar 

  • Lubin D, Jensen EH (1995) Effects of clouds and stratospheric ozone depletion on ultraviolet radiation trends. Nature 377:710–713

    Article  CAS  Google Scholar 

  • Middleton EM, Teramura AH (1993) The role of flavonol glycosides and carotenoids in protecting soybean from ultraviolet-B damage. Plant Physiol 103:741–752

    PubMed  CAS  Google Scholar 

  • Misonou T, Saitoh J, Oshiba S, Tokitomo Y, Maegawa M, Inoue Y, Hori H, Sakurai T (2003) UV-absorbing substance in the red alga Porphyra yezoensis (Bangiales, Rhodophyta) block thymine photodimer production. Mar Biotechnol 5:194–200

    Article  PubMed  CAS  Google Scholar 

  • Mittler R, Tel-Or E (1991) Oxidative stress responses in the unicellular cyanobacterium Synechococcus PCC7942. Free Radic Res Commun 12:845–850

    Article  PubMed  Google Scholar 

  • Nakamura H, Kobayashi J, Hirata Y (1982) Separation of mycosporine-like amino acids in marine organisms using reverse-phase high performance liquid chromatography. J Chromatogr A 250:113–118

    Article  CAS  Google Scholar 

  • Portwich A, Garcia-Pichel F (2000) A novel prokaryotic UVB photoreceptor in the cyanobacterium Chlorogloeopsis PCC 6912. Photochem Photobiol 71:493–498

    Article  PubMed  CAS  Google Scholar 

  • Portwich A, Garcia-Pichel F (2003) Biosynthetic pathway of mycosporines (mycosporine-like amino acids) in the cyanobacterium Chlorogloeopsis sp. strain PCC 6912. Phycologia 42:384–392

    Article  Google Scholar 

  • Schopf JW (2000) The fossil record: tracing the roots of the cyanobacterial lineage. In: Whitton BA, Potts M (eds) The ecology of Cyanobacteria. Their diversity in time and space. Kluwer, Dordrecht, pp 13–35

    Google Scholar 

  • Singh SP, Kumari S, Rastogi RP, Singh KL, Sinha RP (2008) Mycosporine-like amino acids (MAAs): chemical structure, biosynthesis and significance as UV-absorbing/screening compounds. Ind J Exp Biol 46:7–17

    CAS  Google Scholar 

  • Sinha RP, Häder D-P (2002) Life under solar UV radiation in aquatic organisms. Adv Space Res 30:1547–1556

    Article  PubMed  CAS  Google Scholar 

  • Sinha RP, Häder D-P (2008) UV-protectants in cyanobacteria. Plant Sci 174:278–289

    CAS  Google Scholar 

  • Sinha RP, Lebert M, Kumar A, Kumar HD, Häder D-P (1995) Disintegration of phycobilisomes in a rice field cyanobacterium Nostoc sp. following UV irradiation. Biochem Mol Biol Int 37:697–706

    PubMed  CAS  Google Scholar 

  • Sinha RP, Singh N, Kumar A, Kumar HD, Häder M, Häder D-P (1996) Effects of UV irradiation on certain physiological and biochemical processes in cyanobacteria. J Photochem Photobiol B Biol 32:107–113

    Article  CAS  Google Scholar 

  • Sinha RP, Singh N, Kumar A, Kumar HD, Häder D-P (1997) Impacts of ultraviolet-B irradiation on nitrogen-fixing cyanobacteria of rice paddy fields. J Plant Physiol 150:188–193

    CAS  Google Scholar 

  • Sinha RP, Klisch M, Häder D-P (1999) Induction of a mycosporine-like amino acid (MAA) in the rice-field cyanobacterium Anabaena sp. by UV irradiation. J Photochem Photobiol B Biol 52:59–64

    Article  CAS  Google Scholar 

  • Sinha RP, Ambasht NK, Sinha JP, Häder D-P (2003a) Wavelength-dependent induction of a mycosporine-like amino acid in a rice-field cyanobacterium, Nostoc commune: role of inhibitors and salt stress. Photochem Photobiol Sci 2:171–176

    Article  PubMed  CAS  Google Scholar 

  • Sinha RP, Ambasht NK, Sinha JP, Klisch M, Häder D-P (2003b) UV-B-induced synthesis of mycosporine-like amino acids in three strains of Nodularia (cyanobacteria). J Photochem Photobiol B Biol 71:51–58

    Article  CAS  Google Scholar 

  • Sinha RP, Keshari G, Kumari S, Singh SP, Rastogi RP, Singh KL (2006) Screening of mycosporine-like amino acids (MAAs) in cyanobacteria. Modern J Life Sci 5:1–6

    Google Scholar 

  • Sinha RP, Singh SP, Häder D-P (2007) Database on mycosporines and mycosporine-like amino acids (MAAs) in fungi, cyanobacteria, macroalgae, phytoplankton and animals. J Photochem Photobiol B Biol 89:29–35

    Article  CAS  Google Scholar 

  • Smith RC, Prézelin BB, Baker KS, Bidigare RR, Boucher NP, Coley T, Karentz D, MacIntyre S, Matlick HA, Menzies D, Ondrusek M, Wan Z, Waters KJ (1992) Ozone depletion: ultraviolet radiation and phytoplankton biology in Antarctic waters. Science 255:952–959

    Article  PubMed  CAS  Google Scholar 

  • Stanier RY, Cohen-Bazire G (1977) Phototrophic prokaryotes: the cyanobacteria. Annu Rev Microbiol 31:225–274

    Article  PubMed  CAS  Google Scholar 

  • Suh H-J, Lee H-W, Jung J (2003) Mycosporine glycine protects biological systems against photodynamic damage by quenching singlet oxygen with a high efficiency. Photochem Photobiol 78:109–113

    Article  PubMed  CAS  Google Scholar 

  • Vaishampayan A, Sinha RP, Häder D-P, Dey T, Gupta AK, Bhan U, Rao AL (2001) Cyanobacterial biofertilizers in rice agriculture. Bot Rev 67:453–516

    Article  Google Scholar 

  • Vincent WF, Neale PJ (2000) Mechanisms of UV damage to aquatic organisms. In: de Mora SJ, Demers S, Vernet M (eds) The effects of UV radiation on marine ecosystems. Cambridge University Press, Cambridge, pp 149–176

    Google Scholar 

  • Xue L, Zhang Y, Zhang T, An L, Wang X (2005) Effects of enhanced ultraviolet-B radiation on algae and cyanobacteria. Crit Rev Microbiol 31:79–89

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

The authors sincerely acknowledge the excellent technical support by Martin Schuster.

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Authors and Affiliations

  1. Department of Biology, Chair of Plant Ecophysiology, Friedrich-Alexander University Erlangen-Nuremberg, Staudtstrasse 5, 91058, Erlangen, Germany

    Shailendra P. Singh, Manfred Klisch & Donat-P. Häder

  2. Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, 221005, India

    Rajeshwar P. Sinha

Authors
  1. Shailendra P. Singh
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  2. Rajeshwar P. Sinha
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  3. Manfred Klisch
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  4. Donat-P. Häder
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Correspondence to Donat-P. Häder.

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Singh, S.P., Sinha, R.P., Klisch, M. et al. Mycosporine-like amino acids (MAAs) profile of a rice-field cyanobacterium Anabaena doliolum as influenced by PAR and UVR. Planta 229, 225–233 (2008). https://doi.org/10.1007/s00425-008-0822-1

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  • DOI: https://doi.org/10.1007/s00425-008-0822-1

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