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Insight into Types I and II nonhost resistance using expression patterns of defense-related genes in tobacco

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Abstract

Plants protect themselves against pathogens using a range of response mechanisms. There are two categories of nonhost resistance: Type I, which does not result in visible cell death; and Type II, which entails localized programmed cell death (or hypersensitive response) in response to nonhost pathogens. The genes responsible for these two systems have not yet been intensively investigated at the molecular level. Using tobacco plants (Nicotiana tabacum), we compared expression of 12 defense-related genes between a Type I (Xanthomonas axonopodis pv. glycines 8ra) nonhost interaction, and two Type II (Pseudomonas syringae pv. syringae 61 and P. syringae pv. phaseolicola NPS3121) nonhost interactions, as well as those expressed during R gene-mediated resistance to Tobacco mosaic virus. In general, expression of most defense-related genes during R gene-mediated resistance was activated 48 h after challenge by TMV; the same genes were upregulated as early as 9 h after infiltration by nonhost pathogens. Surprisingly, X. axonopodis pv. glycines (Type I) elicited the same set of defense-related genes as did two pathovars of P. syringae, despite the absence of visible cell death. In two examples of Type II nonhost interactions, P. syringae pv. phaseolicola NPS3121 produced an expression profile more closely resembling that of X. axonopodis pv. glycines 8ra, than that of P. syringae pv. syringae 61. These results suggest that Type I nonhost resistance may act as a mechanism providing a more specific and active defense response against a broad range of potential pathogens.

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Fig. 1
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Abbreviations

Ng-CDM1 :

Nicotiana glutinosa-cell death marker1

MAPK:

Mitogen-activated protein kinase

Nt-hsr203J :

Nicotiana tabacum-hypersensitive response related203J

Nt-hin1 :

Nicotiana tabacum-harpin induced1

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Acknowledgements

We thank Ingyu Hwang (Department of Plant Pathology, Seoul National University) for providing three bacterial strains, and Li Kang and Kiran Mysore for critical reading of our manuscript. This research was supported by grants from the Plant Diversity Research Center (PDRC) and the Crop Functional Genomics Center (CFGC) of the 21st Century Frontier Research Program, funded by Ministry of Science and Technology (MOST).

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Authors and Affiliations

  1. Plant Genomics Laboratory, Korea Research Institute of Bioscience and Biotechnology, 305-600, Daejeon, South Korea

    Sang-Keun Oh, Sanghyeob Lee, Eunsook Chung, Jeong Mee Park & Doil Choi

  2. Plant Pathology Laboratory, Chungnam National University, 305-764, Daejeon, South Korea

    Seung Hun Yu

  3. Laboratory of Microbial Genomics, Korea Research Institute of Bioscience and Biotechnology, 305-600, Daejeon, South Korea

    Choong-Min Ryu

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  1. Sang-Keun Oh
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  2. Sanghyeob Lee
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Correspondence to Choong-Min Ryu.

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Oh, SK., Lee, S., Chung, E. et al. Insight into Types I and II nonhost resistance using expression patterns of defense-related genes in tobacco. Planta 223, 1101–1107 (2006). https://doi.org/10.1007/s00425-006-0232-1

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