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Planta

, Volume 221, Issue 6, pp 937–952 | Cite as

Sugar-mediated transcriptional regulation of the Gap gene system and concerted photosystem II functional modulation in the microalga Scenedesmus vacuolatus

  • Federico Valverde
  • José M. Ortega
  • Manuel Losada
  • Aurelio SerranoEmail author
Original Article

ABSTRACT

Partial cDNAs corresponding to the GapA, GapC and GapN genes that encode the three different glyceraldehyde-3-phosphate dehydrogenases (GAPDHs) of the green microalga Scenedesmus vacuolatus SAG 211-8b have been cloned and characterized. Northern blot experiments, as well as immunoblots and activity measurements, demonstrate a differential regulation by sugars of the components of the algal Gap gene system. Addition of glucose or other metabolizable sugars to photoautotrophic cultures promoted a drastic repression of the GapA gene and depletion to negligible levels of the corresponding GAPDHA, a chloroplastic protein involved in photosynthetic CO2 assimilation. By contrast, expression of the GapC and GapN genes encoding their cytosolic counterparts involved in glycolysis was enhanced. However, no down-regulation of the GapA gene by glucose took place in the dark, indicating that the observed effect is associated with sugar assimilation in the light. Likewise, glucose promoted in illuminated algal cultures a severe decrease of photosystem II functionality, estimated by O2 evolution activity, thermoluminescence emission and D1 protein level, while again, no effect was observed in the dark. On the basis of the correlation found between photosystem II performance and sugar transcriptional regulation of the GapA gene, a scenario of sugar-mediated regulation of photosynthetic metabolism in microalgae is proposed that will help to explain the so-called glucose bleaching effect in photosynthetic eukaryotes.

Keywords

GAPDH Photoinhibition Scenedesmus vacuolatus Sugar gene regulation 

Abbreviations

BPGA

1,3-Bisphosphoglycerate

G3P

Glyceraldehyde-3-phosphate

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

PGA

3-Phosphoglycerate

Pi

Inorganic orthophosphate

PSI, II

Photosystem I, II

TL

Thermoluminescence

Notes

Acknowledgments

The authors gratefully thank Dr. Carolyn Silflow (University of Minnesota, MN, USA), Prof. Rafael Picorell (Estación Experimental Aula Dei, CSIC, Zaragoza, Spain) and Prof. Carlos Gómez-Moreno (University of Zaragoza, Spain) for kindly providing the β 2-tubulin cDNA clone of C. reinhardtii and antibodies against D1 and FNR proteins, respectively. This work was supported by Grants PB-97/1135, BMC2001-563 from MCYT (Spanish Government); Grupo CVI-261 of III-PAI (Andalusian Regional Government) and EU project contract MERG-CT-2004-505303.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Federico Valverde
    • 1
  • José M. Ortega
    • 1
  • Manuel Losada
    • 1
  • Aurelio Serrano
    • 1
    Email author
  1. 1.Instituto de Bioquímica Vegetal y FotosíntesisUniversidad de Sevilla-CSICSevilleSpain

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