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A fasciclin-domain containing gene, ZeFLA11, is expressed exclusively in xylem elements that have reticulate wall thickenings in the stem vascular system of Zinnia elegans cv Envy

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Abstract

The vascular cylinder of the mature stem of Zinnia elegans cv Envy contains two anatomically distinct sets of vascular bundles, stem bundles and leaf-trace bundles. We isolated a full-length cDNA of ZeFLA11, a fasciclin-domain-containing gene, from a zinnia cDNA library derived from in vitro cultures of mesophyll cells induced to form tracheary elements. Using RNA in situ hybridization, we show that ZeFLA11 is expressed in the differentiating xylem vessels with reticulate type wall thickenings and adjacent parenchyma cells of zinnia stem bundles, but not in the leaf-trace bundles that deposit spiral thickenings. Our results suggest a function for this cell-surface GPI-anchored glycoprotein in secondary wall deposition during differentiation of metaxylem tissue with reticulate vessels.

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Abbreviations

AGP:

Arabinogalactan protein

FLA:

Fasciclin-like arabinogalactan protein

GPI:

Glycosylphosphatidylinositol

TE:

Tracheary element

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Acknowledgements

Many thanks to Dimitra Milioni for contributing the partial sequence of ZeFLA11, Nicola Stacey, John Innes Centre, for providing zinnia plants and cell cultures, and to JIF-Nuffield fellow, Ranu Dhalla for assistance with RT-PCR. We gratefully acknowledge the financial support of EU EDEN project no. QLK5-CT-2001-00443 (PD), Leverhulme Trust (PD), BBSRC (KR) and The Royal Society (MCM). PD performed the experiments and drafted the manuscript, KF captured SEM images, KR and MCM co-supervised the work and co-edited the manuscript.

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Authors and Affiliations

  1. Department of Cell and Developmental Biology, John Innes Centre, Colney Lane, NR4 7UH, Norwich, UK

    Preeti Dahiya, Kim Findlay, Keith Roberts & Maureen C. McCann

  2. Department of Biological Sciences, Purdue University, West Lafayette, IN, 47907-1392, USA

    Maureen C. McCann

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  1. Preeti Dahiya
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  2. Kim Findlay
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  3. Keith Roberts
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  4. Maureen C. McCann
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Correspondence to Maureen C. McCann.

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Dahiya, P., Findlay, K., Roberts, K. et al. A fasciclin-domain containing gene, ZeFLA11, is expressed exclusively in xylem elements that have reticulate wall thickenings in the stem vascular system of Zinnia elegans cv Envy. Planta 223, 1281–1291 (2006). https://doi.org/10.1007/s00425-005-0177-9

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