Abstract
Legumes can acquire nitrogen through a symbiotic interaction with rhizobial bacteria. The initiation of this process is determined by a molecular dialogue between the two partners. Legume roots exude flavonoids that induce the expression of the bacterial nodulation genes, which encode proteins involved in the synthesis and secretion of signals called Nod factors (NFs). NFs signal back to the plant root and trigger several responses, leading to bacterial invasion and nodule formation. Here, we describe the molecular and cellular characterization of a Phaseolus vulgaris non-nodulating mutant (NN-mutant). Root hair cells of the NN-mutant plant respond with swelling and branching when inoculated with Rhizobium etli, albeit without curling induction. Furthermore, neither initiation of cell division in the outer cortex, nor entrapment of bacteria nor infection thread formation was observed. Both the bean wild-type and the NN-mutant responded with elevated intracellular calcium changes in the root hairs. Although the NN-mutant is deficient in early nodulin gene expression when inoculated with R. etli, it can be effectively colonized by arbuscular mycorrhizal fungi (Glomus intraradices). Our data indicate that the P. vulgaris NN-mutant is not blocked at the NFs early perception stage, but at later downstream stages between Ca2+ signaling and early nodulin induction. This supports the idea that both microsymbionts are perceived and trigger different downstream pathways in the host plant.
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Abbreviations
- CaM:
-
Calmodulin
- HPLC:
-
High-performance liquid chromatography
- CCD:
-
Charge-coupled device
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Acknowledgment
We thank DGAPA (grant IN209202, IN228903 and IN209804) and CONACYT (grant 42560-Q) for the financial support. We also thank Dr. Beatriz Xoconostle for providing us with the G. intraradices strain and Alberto Camas for his helpful technical advices. We thank Dr. Marco Villanueva, Otto Geiger and Chris Wood for their critical reading of the manuscript. We are grateful to the UC-MEXUS CN98-0706 grant for partial support. E. Alemán was supported by a CONACYT scholarship No. 176451.
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Cárdenas, L., Alemán, E., Nava, N. et al. Early responses to Nod factors and mycorrhizal colonization in a non-nodulating Phaseolus vulgaris mutant. Planta 223, 746–754 (2006). https://doi.org/10.1007/s00425-005-0132-9
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DOI: https://doi.org/10.1007/s00425-005-0132-9