Planta

, Volume 223, Issue 2, pp 180–190 | Cite as

Overexpression of Arabidopsis phytochelatin synthase in tobacco plants enhances Cd2+ tolerance and accumulation but not translocation to the shoot

  • Mirella Pomponi
  • Vincenzo Censi
  • Valentina Di Girolamo
  • Angelo De Paolis
  • Luigi Sanità di Toppi
  • Rita Aromolo
  • Paolo Costantino
  • Maura Cardarelli
Original Article

Abstract

Phytochelatins (PCs) are metal binding peptides involved in heavy metal detoxification. To assess whether enhanced phytochelatin synthesis would increase heavy metal tolerance and accumulation in plants, we overexpressed the Arabidopsis phytochelatin synthase gene (AtPCS1) in the non-accumulator plant Nicotiana tabacum. Wild-type plants and plants harbouring the Agrobacterium rhizogenes rolB oncogene were transformed with a 35SAtPCS1 construct. Root cultures from rolB plants could be easily established and we demonstrated here that they represent a reliable system to study heavy metal tolerance. Cd2+ tolerance in cultured rolB roots was increased as a result of overexpression of AtPCS1, and further enhanced when reduced glutathione (GSH, the substrate of PCS1) was added to the culture medium. Accordingly, HPLC analysis showed that total PC production in PCS1-overexpressing rolB roots was higher than in rolB roots in the presence of GSH. Overexpression of AtPCS1 in whole seedlings led to a twofold increase in Cd2+ accumulation in the roots and shoots of both rolB and wild-type seedlings. Similarly, a significant increase in Cd2+ accumulation linked to a higher production of PCs in both roots and shoots was observed in adult plants. However, the percentage of Cd2+ translocated to the shoots of seedlings and adult overexpressing plants was unaffected. We conclude that the increase in Cd2+ tolerance and accumulation of PCS1 overexpressing plants is directly related to the availability of GSH, while overexpression of phytochelatin synthase does not enhance long distance root-to-shoot Cd2+ transport.

Keywords

Cd accumulation Cd tolerance Glutathione PCS1 overexpression Tobacco 

Abbreviations

DAG

Days after germination

GSH

Glutathione

PC

Phytochelatin

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Mirella Pomponi
    • 1
  • Vincenzo Censi
    • 2
    • 6
  • Valentina Di Girolamo
    • 2
  • Angelo De Paolis
    • 3
  • Luigi Sanità di Toppi
    • 4
  • Rita Aromolo
    • 5
  • Paolo Costantino
    • 1
  • Maura Cardarelli
    • 2
  1. 1.Dipartimento di Genetica e Biologia MolecolareUniversitá La SapienzaRomeItaly
  2. 2.Istituto Biologia e Patologia Molecolari, CNRUniversitá La SapienzaRomeItaly
  3. 3.Istituto di Scienze delle Produzioni Alimentari CNR (ISPA)LecceItaly
  4. 4.Dipartimento di Biologia Evolutiva e FunzionaleUniversitá di ParmaParmaItaly
  5. 5.Istituto Sperimentale per la Nutrizione delle PianteRomeItaly
  6. 6.Istituto di Ricerche di Biologia Molecolare P. Angeletti, IRBMPomezia (Rome)Italy

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