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Non-phosphorylating bypass of the plant mitochondrial respiratory chain by stress protein CSP 310

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Abstract

Recently, it has been reported that the cold-stress protein CSP 310, discovered in the cytoplasm of cold-resistant winter cereals, causes uncoupling of oxidative phosphorylation during cold stress. To understand how the uncoupling mechanism of CSP differs from that of cyanide-insensitive alternative oxidase and plant mitochondrial uncoupling protein, we determined the effect of respiratory-chain inhibition on winter wheat (Triticum aestivum L. cv. Zalarinka) mitochondria. Our data show a possible involvement of stress protein CSP 310 in mitochondrial electron transport in winter wheat. CSP 310 shunts electrons around the main cytochrome pathway of the mitochondrial respiratory chain, i.e. electron flow bypasses ubiquinone and complex III via CSP 310 to complex IV.

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Abbreviations

AOX :

Alternative oxidase

BHAM :

Benzohydroxamic acid

CSP :

Cold-shock protein

DPI :

Diphenyleneiodonium chloride

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Acknowledgements

This work was supported, in part, by the Russian Science Support Foundation, the Russian Foundation of Basic Research (project 03-04-48151) and the Siberian Division of Russian Academy of Sciences Youth Grant (project 78). We thank Professor J. Wiskich (School of Biological Sciences, Flinders University of South Australia, Australia) and anonymous referees for helpful discussions and editorial help.

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Authors and Affiliations

  1. Siberian Institute of Plant Physiology and Biochemistry, Russian Academy of Sciences, Irkutsk-33, P.O. Box 1243, 664033, Irkutsk, Russia

    A. V. Kolesnichenko, O. I. Grabelnych, T. P. Pobezhimova & V. K. Voinikov

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  1. A. V. Kolesnichenko
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  2. O. I. Grabelnych
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  3. T. P. Pobezhimova
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  4. V. K. Voinikov
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Correspondence to A. V. Kolesnichenko.

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Kolesnichenko, A.V., Grabelnych, O.I., Pobezhimova, T.P. et al. Non-phosphorylating bypass of the plant mitochondrial respiratory chain by stress protein CSP 310. Planta 221, 113–122 (2005). https://doi.org/10.1007/s00425-004-1419-y

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  • DOI: https://doi.org/10.1007/s00425-004-1419-y

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