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Planta

, Volume 220, Issue 3, pp 424–433 | Cite as

Abscisic acid-inducible nuclear proteins bind to bipartite promoter elements required for ABA response and embryo-regulated expression of the carrot Dc3 gene

  • Hwa-Jee Chung
  • Hong Yong Fu
  • Terry L. ThomasEmail author
Original Article

Abstract

The carrot (Daucus carota L.) lea-class gene Dc3 is expressed in developing seeds and in vegetative tissues subject to drought and treatment with exogenous abscisic acid (ABA). Cis regulatory elements involved in seed-specific expression and in response to ABA were identified in transgenic tobacco (Nicotiana tabacum L.) using β-glucuronidase (GUS) reporter gene constructs containing a series of deletion and orientation mutants of the Dc3 promoter. These experiments demonstrated that the Dc3 promoter is comprised of a proximal promoter region (PPR) and a distal promoter region (DPR). TCGTGT motifs in the DPR in combination with the PPR comprise a novel, bipartite ABA module in the Dc3 gene promoter. The PPR contains cis-acting elements responsible for the developmental regulation of Dc3 expression in seeds. Five similar sequence motifs with the consensus ACACgtGCa were identified in the PPR. Both DPR and PPR interact with common nuclear proteins that are present in embryos and are inducible by ABA in vegetative tissues.

Keywords

Abscisic acid Cis regulatory element Daucus Seed development Transcription factor 

Abbreviations

ABA

Abscisic acid

ABRE

ABA-response element

bZIP

Basic leucine zipper

DAR

Distal ABA-response region

DPBF

Dc3 promoter-binding factor

DPR

Distal promoter region

GMSA

Gel mobility-shift assay

GUS

β-Glucuronidase

LEA

Late embryogenesis abundant

PPR

Proximal promoter region

Notes

Acknowledgement

This work was financially supported in part by USDA NRICGP grants (Nos. 94373041228, 97353044552, and 013530410940).

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Hwa-Jee Chung
    • 2
  • Hong Yong Fu
    • 3
  • Terry L. Thomas
    • 1
    Email author
  1. 1.Department of BiologyTexas A&M UniversityCollege StationUSA
  2. 2.Laboratory of Functional Genomics for Plant Secondary MetabolismEugentech Inc.DaejonKorea
  3. 3.Institute of BotanyAcademia SinicaTaipeiTaiwan, R.O.C.

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