Maternal synthesis of abscisic acid controls seed development and yield in Nicotiana plumbaginifolia
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The role of maternally derived abscisic acid (ABA) during seed development has been studied using ABA-deficient mutants of Nicotiana plumbaginifolia Viviani. ABA deficiency induced seed abortion, resulting in reduced seed yield, and delayed growth of the remaining embryos. Mutant grafting onto wild-type stocks and reciprocal crosses indicated that maternal ABA, synthesized in maternal vegetative tissues and translocated to the seed, promoted early seed development and growth. Moreover ABA deficiency delayed both seed coat pigmentation and capsule dehiscence. Mutant grafting did not restore these phenotypes, indicating that ABA synthesized in the seed coat and capsule envelope may have a positive effect on capsule and testa maturation. Together these results shed light on the positive role of maternal ABA during N. plumbaginifolia seed development.
KeywordsAbscisic acid Dormancy Mutant Nicotiana Seed
Days after pollination
We thank Helen North and Isabelle Debeaujon for their critical reading of the manuscript. We also thank Krystyna Gofron and Michel Lebrusq for technical assistance with plant culture.
- Cheng WH, Endo A, Zhou L, Penney J, Chen HC, Arroyo A, Leon P, Nambara E, Asami T, Seo M, Koshiba T, Sheen J (2002) A unique short-chain dehydrogenase/reductase in Arabidopsis glucose signaling and abscisic acid biosynthesis and functions. Plant Cell 14:2723–2743Google Scholar
- Duckham SC, Lindforth RST, Taylor IB (1991) Abscisic acid-deficient mutants at the aba gene locus of Arabidopsis thaliana are impaired in the epoxidation of zeaxanthin. Plant Cell Environ 14:601–606Google Scholar
- Hilhorst HWM, Downie B (1995) Primary dormancy in tomato (Lycopersicon esculentum cv. Moneymaker): studies with the sitiens mutant. J Exp Bot 47:89–97Google Scholar
- Karssen CM, Brinkhorst-Van der Swan DLC, Breekland AD, Koornneef M (1983) Induction of seed dormancy during seed development by endogenous abscisic acid: studies on abscisic acid-deficient genotypes of Arabidopsis thaliana L. Heynh. Planta 157:158–165Google Scholar
- Koornneef M, Hanhart CJ, Hilhorst HWM, Karssen CM (1989) In vivo inhibition of seed development and reserve protein accumulation in recombinants of abscisic acid biosynthesis and responsiveness mutants in Arabidopsis thaliana. Plant Physiol 90:463–469Google Scholar
- Kraepiel Y, Rousselin P, Sotta B, Kerhoas L, Einhorn J, Caboche M, Miginiac E (1994) Analysis of phytochrome- and ABA-deficient mutants suggests that ABA degradation is controlled by light in Nicotiana plumbaginifolia. Plant J 6:665–672Google Scholar
- Marin E, Marion-Poll A (1997) Tomato flacca mutant is impaired in ABA aldehyde oxidase and xanthine dehydrogenase activities. Plant Physiol Biochem 35:369–372Google Scholar
- Marin E, Nussaume L, Quesada A, Gonneau M, Sotta B, Hugueney P, Frey A, Marion-Poll A (1996) Molecular identification of zeaxanthin epoxidase of Nicotiana plumbaginifolia, a gene involved in abscisic acid biosynthesis and corresponding to the ABA locus of Arabidopsis thaliana. EMBO J 15:2331–2342PubMedGoogle Scholar
- Meurs C, Basra AS, Karssen CM, van Loon LC (1992) Role of abscisic acid in the induction of desiccation tolerance in developing seeds of Arabidopsis thaliana. Plant Physiol 98:1484–1493Google Scholar
- Rock CD, Quatrano RS (1995) The role of hormones during seed development. In: Davies PJ (eds) Plant hormones. Kluwer, Dordrecht, pp 671–697Google Scholar