Abstract
Polygalacturonase (PG) is a cell wall-associated protein that degrades pectin. A ZePG1 cDNA encoding a putative PG was isolated from Zinnia elegens L. and a rabbit antibody specific to the ZePG1 protein was generated. The level of the ZePG1 protein was up-regulated when tracheary element differentiation was initiated. Using gold-labeled secondary antibodies for light and electron microscopy, ZePG1 protein was localized in cultured Zinnia cells. This protein was preferentially distributed on tracheary elements (TEs). At the subcellular level, the protein was localized on secondary wall thickenings, primary walls, Golgi bodies and vesicles. Thus, the putative role of the ZePG1 protein might be the degradation of pectic substances before lignification. Some non-TE cells also accumulated ZePG1 protein on primary walls, Golgi bodies and vesicles. The accumulation of ZePG1 protein on primary walls seems to be at the elongating tips of non-TE cells. In plants, ZePG1 protein was localized on the secondary wall thickenings of differentiating TEs and phloem regions. These results suggest that the expression of the ZePG1 protein is highly regulated both spatially and temporally during in vitro and in situ TE differentiation.
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Abbreviations
- GST :
-
Glutathione-S-transferase
- PATAg :
-
Periodic acid–thiocarbohydrazide–silver proteinate
- PG :
-
Polygalacturonase
- TE :
-
Tracheary element
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Acknowledgements
The authors express their sincere thanks to Dr. Dwight K. Romanovicz for kindly reading this manuscript and providing valuable suggestions. The authors express their special thanks to Prof. Roland M. Brown Jr. for reading this manuscript and providing helpful comments. The authors also thank Dr. Munetaka Sugiyama for supplying ZePG1 cDNA and sharing his unpublished data. This work was supported in part by Grants-in-Aid from the Ministry of Education, Science, Sports, and Culture of Japan (Nos. 10219201 and 14036205 to H.F.), and from the Japan Society for the Promotion of Science (13440236 to H.F.), from the Mitsubishi Foundation to H.F., and by the Japan Society for the Promotion of Science Research Fellowships for Young Scientists (to N.S.).
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Nakashima, J., Endo, S. & Fukuda, H. Immunocytochemical localization of polygalacturonase during tracheary element differentiation in Zinnia elegans . Planta 218, 729–739 (2004). https://doi.org/10.1007/s00425-003-1167-4
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DOI: https://doi.org/10.1007/s00425-003-1167-4