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The slow wound-response of γVPE is regulated by endogenous salicylic acid in Arabidopsis

Planta volume 218, pages 599–605 (2004)Cite this article

Abstract

Vacuolar processing enzyme (VPE) is a cysteine protease responsible for the maturation of various vacuolar proteins in higher plants. The Arabidopsis thaliana (L.) Heynh. γVPE gene, encoding a VPE homologue, is slowly up-regulated in both local and systemic leaves in response to wounding. To clarify the activation mechanism of γVPE, we examined the accumulation of γVPE mRNA after hormone treatments or after wounding in wild-type and various mutant plants of Arabidopsis. Both ethylene and jasmonic acid (JA) are known as signal molecules that activate the wound-responsive genes. However, treatment with exogenous JA had little effect on the γVPE response, although JA activated the vegetative storage protein (VSP) gene, a typical wound-responsive gene. Wounding activated γVPE even in two ethylene-insensitive plants (etr1-1 and ein2-1). Thus, the wound-induced expression of γVPE was independent of ethylene and JA. We found that the wound-induced expression of γVPE was reduced in two SA-deficient plants (pad4-1 and NahG), while the wound-induced expression of VSP increased in these mutants. Appreciable accumulation of SA was not observed in either the local or systemic leaves after wounding. These results suggest that endogenous SA enhances the wound-induced expression of γVPE and attenuates the wound-induced expression of VSP, although SA is not a wound-signal that directly activates these genes.

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Fig. 1a–c
Fig. 2a–c
Fig. 3a–e
Fig. 4

Abbreviations

ABA:

abscisic acid

GST:

glutathione S-transferase

INA:

2,6-dichloroisonicotinic acid

JA:

jasmonic acid

MeJA:

methyl jasmonate

PR:

pathogenesis-related

RBCS:

Rubisco small subunit

SA:

salicylic acid

VPE:

vacuolar processing enzyme

VSP:

vegetative storage protein

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Acknowledgements

We are grateful to Syngenta Corporation for providing the NahG transgenic plant, and to the Arabidopsis Biological Resource Center for providing Arabidopsis mutant plants and EST clones.This work was supported by CREST of the Japan Science and Technology Corporation, and Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (nos. 12138205 and 12304049), and for a postdoctoral fellowship to K.Y. from the Japan Society for the Promotion of Science.

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Affiliations

  1. Department of Botany, Graduate School of Science, Kyoto University, 606-8502, Kyoto, Japan

    Kenji Yamada & Ikuko Hara-Nishimura

  2. Department of Cell Biology, National Institute for Basic Biology, 444-8585, Okazaki, Japan

    Mikio Nishimura

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  1. Kenji Yamada
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  2. Mikio Nishimura
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  3. Ikuko Hara-Nishimura
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Correspondence to Ikuko Hara-Nishimura.

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Yamada, K., Nishimura, M. & Hara-Nishimura, I. The slow wound-response of γVPE is regulated by endogenous salicylic acid in Arabidopsis . Planta 218, 599–605 (2004). https://doi.org/10.1007/s00425-003-1131-3

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Keywords

  • Arabidopsis
  • Jasmonic acid
  • Salicylic acid
  • Vacuolar processing enzyme
  • Vegetative storage protein
  • Wounding