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Differential Al resistance and citrate secretion in barley (Hordeum vulgare L.)

Planta volume 217, pages 794–800 (2003)Cite this article

Abstract

While barley (Hordeum vulgare L.) is the most sensitive species to Al toxicity among small-grain crops, variation in Al resistance between cultivars does exist. We examined the mechanism responsible for differential Al resistance in 21 barley varieties. Citrate was secreted from the roots in response to Al stress. A positive correlation between citrate secretion and Al resistance [(root elongation with Al)/(root elongation without Al)] and a negative correlation between citrate secretion and Al content of root apices, were obtained, suggesting that citrate secretion from the root apices plays an important role in excluding Al and thereby detoxifying Al. The Al-induced secretion of citrate was characterized using an Al-resistant variety (Sigurdkorn) and an Al-sensitive variety (Kearney). In Sigurdkorn, Al-induced secretion of citrate occurred within 20 min, and the secretion did not increase with increasing external Al concentration. The Al-induced citrate secretion ceased at low temperature (6 °C) and was inhibited by anion-channel inhibitors. Internal citrate content of root apices was increased by Al exposure in Sigurdkorn, but was not affected in Kearney. The activity of citrate synthase was unaffected by Al in both Al-resistant and Al-sensitive varieties. The secretion rate of organic acid anions from barley was the lowest among wheat, rye and triticale.

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Abbreviations

CS:

citrate synthase

NIF:

niflumic acid

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Acknowledgements

This study was supported in part by a Grant-in-Aid for General Scientific Research (Grant No. 13660067 to J.F. Ma) from the Ministry of Education, Science, Sports and Culture of Japan, by CREST, JST (Japan Science and Technology Cooperation), and NSFC (No. 30228023 to J.F. Ma). We thank Julie Hayes for her critical reading of this manuscript.

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Affiliations

  1. Faculty of Agriculture, Kagawa University, Ikenobe 2393, Miki-cho, Kita-gun, 761-0795, Kagawa, Japan

    Zhuqing Zhao & Jian Feng Ma

  2. Research Institute for Bioresources, Okayama University, Chuo 2-20-1, 710-0046, Kurashiki, Japan

    Kazuhiro Sato & Kazuyoshi Takeda

  3. College of Resources and Environment, Huazhong Agricultural University, 430070, Wuhan, China

    Zhuqing Zhao

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  1. Zhuqing Zhao
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  2. Jian Feng Ma
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  3. Kazuhiro Sato
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Correspondence to Jian Feng Ma.

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Zhao, Z., Ma, J.F., Sato, K. et al. Differential Al resistance and citrate secretion in barley (Hordeum vulgare L.). Planta 217, 794–800 (2003). https://doi.org/10.1007/s00425-003-1043-2

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  • DOI: https://doi.org/10.1007/s00425-003-1043-2

Keywords

  • Aluminium
  • Hordeum
  • Citrate
  • Metabolism
  • Secretion