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Two Brassica napus polygalacturonase inhibitory protein genes are expressed at different levels in response to biotic and abiotic stresses

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Abstract

Plants encode a distinct set of polygalacturonase inhibitory proteins (PGIPs) that function to inhibit polygalacturonase enzymes produced by soft-rot fungal pathogens. We characterized two PGIP-encoding genes (Bnpgip1 and Bnpgip2) from Brassica napus DH12075 (a double-haploid line derived from a cross between 'Crésor' and 'Westar'). The two proteins exhibit 67.4% identity at the amino acid level and contain 10 imperfect leucine-rich repeats. The pgip genes are present as a small multigene family in B. napus with at least four members. Bnpgip1 and Bnpgip2 are constitutively expressed in roots, stems, flower buds and open flowers. In mature leaf tissue, different levels of induction were observed in response to biotic and abiotic stresses. Bnpgip1 expression was highly responsive to flea beetle feeding and mechanical wounding, weakly responsive to Sclerotinia sclerotiorum infection and exposure to cold but not to dehydration. Conversely, Bnpgip2 expression was strongly induced by S. sclerotiorum infection and to a lesser degree by wounding but not by flea beetle feeding. Application of jasmonic acid to leaves induced both Bnpgip1 and Bnpgip2 gene expression; however, salicylic acid did not activate either gene. Taken together, these results suggest that separate pathways regulate Bnpgip1 and Bnpgip2, and that their roles in plant development or resistance to biotic and abiotic stress differ.

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Abbreviations

EST:

expressed sequence tag

JA:

jasmonic acid

LRR:

leucine-rich repeat

PG:

polygalacturonase

PGIP:

polygalacturonase inhibitory protein

SA:

salicylic acid

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Acknowledgements

We thank the Saskatchewan Agriculture Development Fund, the Canola Council of Canada and the Federal Matching Investments Initiative for providing funding to support this work and Branimir Gjetvaj for EST sequencing.

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  1. Molecular Genetics Section, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK S7N 0X2, Canada

    Rugang Li, Roger Rimmer, Min Yu, Andrew G. Sharpe, Ginette Séguin-Swartz, Derek Lydiate & Dwayne D. Hegedus

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  1. Rugang Li
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  2. Roger Rimmer
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Correspondence to Dwayne D. Hegedus.

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Li, R., Rimmer, R., Yu, M. et al. Two Brassica napus polygalacturonase inhibitory protein genes are expressed at different levels in response to biotic and abiotic stresses. Planta 217, 299–308 (2003). https://doi.org/10.1007/s00425-003-0988-5

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