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Enhancement of cold tolerance and inhibition of lipid peroxidation by citrus dehydrin in transgenic tobacco

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Abstract

Citrus (Citrus unshiu Marcov.) dehydrin in response to chilling stress was overexpressed in tobacco (Nicotiana tabacum L.), and the cold stress tolerance of transgenics at low temperature was analyzed. The freezing at −4 °C for 3 h of 24 independent lines indicated that a phenotype expressing citrus dehydrin showed less electrolyte leakage than the control. Dehydrin protein content was correlated with freezing tolerance in transgenics. Dehydrin-expressing tobacco exhibited earlier germination and better seedling growth than the control at 15 °C. Cell fractionation experiments suggested that the protein was predominantly expressed in mitochondria and the soluble fraction. Malondialdehyde production enhanced by chilling stress was lower in tobacco plants expressing citrus dehydrin than in control phenotypes. Dehydrin protein, purified from Escherichia coli expressing citrus dehydrin cDNA, prevented peroxidation of soybean (Glycine max L.) liposomes in vitro. The inhibitory activity of dehydrin against liposome oxidation was stronger than that of albumin, glutathione, proline, glycine betaine, and sucrose. These results suggest that dehydrin facilitates plant cold acclimation by acting as a radical-scavenging protein to protect membrane systems under cold stress.

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Abbreviations

AAPH:

2, 2′-azobis (2-amidinopropane)

BSA:

bovine serum albumin

CuCOR19:

Citrus unshiu cold-regulated gene encoding a 19-kDa protein

GUS:

β-glucuronidase

MDA:

malondialdehyde

pBIT:

GUS control tobacco

pC19T:

CuCOR19-expressing tobacco

WT:

wild-type tobacco

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Acknowledgement

This study was supported in part by a Grant-in-Aid (No. 12460078) for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan.

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Authors and Affiliations

  1. Faculty of Agriculture, Shizuoka University, 836 Ohya, 422-8529, Shizuoka, Japan

    Masakazu Hara, Shogo Terashima, Tomoko Fukaya & Toru Kuboi

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  1. Masakazu Hara
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  2. Shogo Terashima
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  3. Tomoko Fukaya
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  4. Toru Kuboi
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Correspondence to Masakazu Hara.

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Hara, M., Terashima, S., Fukaya, T. et al. Enhancement of cold tolerance and inhibition of lipid peroxidation by citrus dehydrin in transgenic tobacco. Planta 217, 290–298 (2003). https://doi.org/10.1007/s00425-003-0986-7

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