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Interaction between proliferating cell nuclear antigen and JUN-activation-domain-binding protein 1 in the meristem of rice, Oryza sativa L.

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Abstract

The eukaryotic polymerase processivity factor, proliferating cell nuclear antigen (PCNA), interacts with many cell cycle-regulator proteins and with proteins involved in the mechanisms of DNA replication and repair. In the present study using two-hybrid analysis with PCNA from rice, Oryza sativa L. cv. Nipponbare (OsPCNA), we found that OsPCNA interacted in vitro and in vivo with rice JUN-activation-domain-binding protein 1 (OsJab1), which is known as COP9/signalsome subunit 5. Both OsPCNA and OsJab1 transcripts were expressed strongly in the shoot apical meristem and weakly in young leaves, flag leaves, ears, roots and root tips. No expression was detected in the mature leaves. The OsPCNA and OsJab1 proteins were expressed and accumulated mostly in the shoot apical meristem and ears, suggesting that OsJab1 is involved in cell proliferation in cooperation with OsPCNA. The role of OsPCNA with OsJab1 in plant DNA proliferation is discussed.

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Abbreviations

CSN:

COP9 signalsome

GST:

glutathione S-transferase

PCNA:

proliferating cell nuclear antigen

Jab1:

JUN-activation-domain-binding protein 1

OsPCNA:

Oryza sativa proliferating cell nuclear antigen

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Authors and Affiliations

  1. Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-ken, Japan

    Taichi Yamamoto, Seisuke Kimura, Yoko Mori, Yukinobu Uchiyama, Toyotaka Ishibashi & Kengo Sakaguchi

  2. National Institute of Agrobiological Resources, Tsukuba-shi, 305, Ibaraki-ken, Japan

    Junji Hashimoto

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  1. Taichi Yamamoto
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  2. Seisuke Kimura
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  3. Yoko Mori
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  4. Yukinobu Uchiyama
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  5. Toyotaka Ishibashi
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  6. Junji Hashimoto
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  7. Kengo Sakaguchi
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Correspondence to Kengo Sakaguchi.

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Yamamoto, T., Kimura, S., Mori, Y. et al. Interaction between proliferating cell nuclear antigen and JUN-activation-domain-binding protein 1 in the meristem of rice, Oryza sativa L.. Planta 217, 175–183 (2003). https://doi.org/10.1007/s00425-003-0981-z

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