Ca²⁺ channel inhibition induced by nitric oxide in rat insulinoma RINm5F cells

Abstract

 The effect of nitric oxide (NO) donors on high-voltage-activated Ca²⁺ channels in insulin-secreting RINm5F cells was investigated using the patch-clamp technique in the whole-cell configuration. Sodium nitroprusside (SNP, 2–400 µM) induced a dose-dependent reduction in Ba²⁺ currents with maximal inhibition of 58%. The IC₅₀ for SNP was 45 µM. A different NO donor, (±)S-nitroso-N-acetylpenicillamine (SNAP, 500 µM), also produced a 50% decrease in current amplitude. When 200 µM SNP was administered together with the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidozoline-1-oxyl-3-oxide (carboxy-PTIO, 300 µM), the Ba²⁺ current inhibition was lowered to 7%. Administration of 500 µM 8-bromoguanosine 3′:5′-cyclic monophosphate sodium salt (8-Br-cGMP) mimicked the effects of SNP, causing a comparable decrease (56%) in peak-current amplitude. When soluble guanylyl cyclase was blocked by 10 µM 1H-[1,2,4]oxadiazole[4,3-a]quinoxalin-1-one (ODQ), the inhibitory effect of 200 µM SNP was reduced from 39% to 15%. The SNP-induced current decrease was 36% of controls after the blockade of L-type Ca²⁺ channels and 30% in the presence of 2.5 µM ω-conotoxin-MVIIC. These data indicate that NO inhibits both L-type and P/Q-type Ca²⁺ channels in RINm5F cells, probably by an increase in the intracellular levels of cGMP. NO may then significantly influence the Ca²⁺-dependent release of hormones from secretory cells as well as that of neurotransmitters from nerve terminals.