Chloralose/ketamine anaesthesia preserves a form of postprandial sodium chloride balance in Wistar rats


Studies on the mechanisms underlying Na balance in anaesthetized rats are complicated by the fact that the most frequently used barbiturate anaesthetics attenuate or abolish this phenomenon. In the present study we show that a combination of nonbarbiturate anaesthetics: chloralose (140 mg/kg i.v.) and ketamine (30 mg/kg i.m.), preserve the ability of rats to excrete intragastrically applied NaCl loads dose dependently. Thus rats anaesthetized with this regime excreted 86–102% of intragastrically applied NaCl whereas rats anaesthetized with thiobutabarbitone sodium (Inactin) excreted only 20–28%. We conclude that chloralose/ketamine anaesthesia is suitable for studies on Na balance mechanisms.

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  1. 1.

    Carvalho JS, Cherkes JK (1982) Renin release by pentobarbital anesthesia in the rat: a role for vascular mechanisms. Life Sci 30: 887–897

    PubMed  Article  CAS  Google Scholar 

  2. 2.

    Christensen P, Kristensen LO, Leyssac PP (1973) The effects of amytal and Inactin on isosmotic net fluid transport in the rabbit gall bladder in vitro. Acta Physiol Scand 87: 455–464

    PubMed  Article  CAS  Google Scholar 

  3. 3.

    Fromm M, Oelkers W, Hegel U (1983) Time course of aldosterone and corticosterone plasma levels in rats during general anaesthesia and abdominal surgery. Pflügers Arch 399: 249–254

    PubMed  Article  CAS  Google Scholar 

  4. 4.

    Führ J, Kaczmarczyk J, Krüttgen CD (1955) Eine einfache colorimetrische Methode zur Inulinbestimmung für Nieren-Clearance-Untersuchungen bei Stoffwechselgesunden und Diabetikern. Klin Wochenschr 33: 729–730

    PubMed  Article  Google Scholar 

  5. 5.

    Green CJ (1987) Anaesthesia and analgesia. In: Tiffany AA (ed) Laboratory animals: an introduction for new experimenters (John Wiley and Sons, Chichester, pp 261–302

    Google Scholar 

  6. 6.

    Häberle DA, Ruhland G (1976) Inactin concentrations in plasma of rat during anaesthesia and the effect of this concentration on short circuit current of isolated frog skin. Pflügers Arch 365: 77–80

    PubMed  Article  Google Scholar 

  7. 7.

    Hackenthal E, Hackenthal R, Hofbauer KG (1977) No evidence for product inhibition of the renin angiotensinogen reaction in the rat. Circ Res 41 (Suppl2):49–54

    PubMed  CAS  Google Scholar 

  8. 8.

    Knight TF, Sansom S, Hawk L, Frankfurt SJ, Weinman EJ (1978) The effects of anesthesia on the excretion of an isotonic saline load in the rat. Pflügers Arch 373: 139–143

    PubMed  Article  CAS  Google Scholar 

  9. 9.

    Koeppen BM, Katz AI, Lindheimer MD (1979) Effect of general anaesthesia on renal haemodynamics in the rat. Clin Sci 57: 469–471

    PubMed  CAS  Google Scholar 

  10. 10.

    Kudo M, Kudo T, Matsuki A, Ishihara H (1993) [Effects of ketamine on pituitaryadrenal axis in rats]. Masui 42: 552–556

    PubMed  CAS  Google Scholar 

  11. 11.

    Maddox DA, Price DC, Rector FCJ (1977) Effects of surgery on plasma volume and salt and water excretion in rats. Am J Physiol 233: F600-F606

    PubMed  CAS  Google Scholar 

  12. 12.

    Rutledge JC, Rabinowitz L (1987) Kaliuretic regulatory factors in the rat. Am J Physiol 253: F1182-F1196

    PubMed  CAS  Google Scholar 

  13. 13.

    Tanaka K, Pettinger WA (1974) Renin release and ketamine-induced cardiovascular stimulation in the rat. J Pharmacol Exp Ther 188:229–233

    PubMed  CAS  Google Scholar 

  14. 14.

    Terragno NA, Terragno DA, McGiff JC (1977) Contribution of prostaglandins to the renal circulation in conscious, anesthetized, and laparotomized dogs. Circ Res 40: 590–595

    PubMed  CAS  Google Scholar 

  15. 15.

    Tucker BJ, Peterson W, Ziegler MG, Blantz RC (1982) Analysis of adrenergic effects of the anesthetics Inactin and alpha-chloralose. Am J Physiol 243: F253-F259

    PubMed  CAS  Google Scholar 

  16. 16.

    Walker LA, Buscemi-Bergin M, Gellai M (1983) Renal hemodynamics in conscious rats: effect of anesthesia, surgery, and recovery. Am J Physiol 245: F67-F74

    PubMed  CAS  Google Scholar 

  17. 17.

    Yun JC, Donahue JJ, Bartter FC, Kelly GD (1979) Effect of pentobarbital anesthesia and laparotomy on plasma renin activity in the dog. Can J Physiol Pharmacol 57: 412–416

    PubMed  CAS  Google Scholar 

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Correspondence to D. A. Häberle.

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Metz, C.J.S., Ise, T. & Häberle, D.A. Chloralose/ketamine anaesthesia preserves a form of postprandial sodium chloride balance in Wistar rats. Pflügers Arch. 432, 944–947 (1996).

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Key words

  • Anaesthesia
  • Chloralose
  • Ketamine
  • Sodium excretion
  • Rats