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Simultaneous recording of ATP-sensitive K+ current and intracellular Ca2+ in anoxic rat ventricular myocytes. Effects of glibenclamide

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  • Heart, circulation, respiration and blood; environmental and exercise physiology
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Abstract

We investigated the temporal relationship between the adenosine triphosphate-sensitive K current (Katp current), hypoxic shortening and Ca accumulation in cardiomyocytes exposed to anoxia or metabolic inhibition. Whole-cell, patch-clamp experiments were performed with nonstimulated isolated rat heart ventricular muscle cells loaded with the Ca-sensitive fluorescent dye l-[2-(5-carboxyoxazol-2-yl)-6-amino-benzofuran-5-oxy]-2-(2′-amino-5′-methylphenoxy) ethane-N,N,N′,N′-tetraacetic acid (fura-2) via the patch pipette. After approximately 8 min anoxia, the K ATP current started to rise and reached a maximum of 21.3 ± 3.7 nA (n = 5, recorded at 0 mV clamp potential) within 1–3 min. At that time hypoxic contracture also occurred. Resting cytoplasmic free calcium (Cai) did not change significantly before hypoxic shortening. After hypoxic contracture, the KATP current decreased and Cai started to rise, reaching about 1 μmol/l. The presence of glibenclamide (10μmol/l) in the bath reduced the anoxia-induced KATP current by more than 50%, but did not significantly influence the time dependence of current, hypoxic shortening and Cai, or the magnitude of Cai. Metabolic inhibition with 1.5 mmol/1 CN resulted in KATP current increase and hypoxic shortening, occurring somewhat earlier than under anoxia, but all other parameters were comparable. In non-patch-clamped cells loaded with fura-2 AM ester and field-stimulated with 1 Hz, 1 μmol/1 glibenclamide had no significant effect on the magnitude of the Cai increase caused by exposure of the cells to 1.5 mmol/1 CN-. After CN- wash-out in non-patch-clamped cells, Cai declined, oscillated and finally returned to control values. It can be concluded that glibenclamide inhibits anoxia-induced KATP currents only partially and has no significant effect on anoxiainduced rise in resting Cai.

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  1. Hoechst AG, Cardiovascular Agents, H 821, D-65926, Frankfurt/Main, Germany

    U. Ruß, H. Englert, B. A. Schölkens & H. Gögelein

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  1. U. Ruß
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  2. H. Englert
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  3. B. A. Schölkens
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  4. H. Gögelein
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Ruß, U., Englert, H., Schölkens, B.A. et al. Simultaneous recording of ATP-sensitive K+ current and intracellular Ca2+ in anoxic rat ventricular myocytes. Effects of glibenclamide. Pflügers Arch — Eur J Physiol 432, 75–80 (1996). https://doi.org/10.1007/s004240050107

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  • DOI: https://doi.org/10.1007/s004240050107

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