Abstract
The X-linked Gy mutation is closely linked, but not allelic, to Hyp and is characterized by rickets, hypophosphatemia, decreased renal tubular maximum for phosphate (Pi) reabsorption (TmP) and a specific reduction in renal brush-border membrane (BBM) Na+-Pi cotransport. Gy mice, like their normal litter-mates, respond to a low-Pi diet with an increase in BBM Na+-Pi cotransport, but fail to show an adaptive increase in Tmp. Using an antibody raised against the NH2 terminal peptide of the rat renal-specific Na+-Pi cotransporter (NaPi-2) and a NaPi-2 cDNA probe, we examined the effect of the Gy mutation and low-Pi diet (0.03% Pi) on NaPi-2 protein and mRNA abundance. The reduction in BBM Na+-Pi cotransport in Gy mice (51 ± 5% of normal, P < 0.05) was associated with a decrease in NaPi-2 protein (46 ± 12% of normal, P < 0.05) and mRNA abundance (76 ± 5%, P < 0.05). The low-Pi diet elicited a two-to three-fold increase in Na+-Pi cotransport in both normal and Gy mice that was accompanied by a large increase in NaPi-2 protein (10.2-fold in normal and 16.9-fold in Gy mice) and a modest increase in NaPi-2 mRNA (1.3-fold in both mouse strains, P < 0.05). The present data demonstrate that (1) the renal defect in BBM Pi transport in Gy mice can be ascribed to a deficit in NaPi-2 protein and mRNA abundance, (2) both normal and Gy mice respond to low Pi with an adaptive increase in NaPi-2 protein that exceeds the increase in Na+-Pi cotransport activity and NaPi-2 mRNA, (3) the adaptive increase in NaPi-2 protein and mRNA are not sufficient for the overall increase in TmP following Pi restriction.
Similar content being viewed by others
References
Caverzasio J, Faundez R, Fleisch H, Bonjour J-P (1981) Tubular adaptation to Pi restriction in hypophysectomized rats. Pflügers Arch 392:17–21
Corbeil D, Milhiet P, Simon V, Ingram J, Kenny AJ, Boileau G, Crine P (1993) Rat endopeptidase-24.18 a subunit is secreted into the culture medium as a zymogen when expressed by COS-1 cells. FEBS Lett 335:361–366
Custer M, Lötscher M, Biber J, Murer H, Kaissling B (1994) Expression of Na-Pi cotransport in rat kidney: localization by RT-PCR and immunohistochemistry. Am J Physiol 266:F767-F774
Eicher EM, Southard JL, Scriver CR, Glorieux FH (1976) Hypophosphatemia: mouse model for human familial hypophosphatemic (vitamin D-resistant) rickets. Proc Natl Acad Sci USA 73:4667–4671
Gray RW, Napoli JL (1983) Dietary phosphate deprivation increases 1,25-dihydroxyvitamin D3 synthesis in rat kidney in vitro. J Biol Chem 258:1152–1155
Greger R, Lang F, Marchand G, Knox FG (1977) Site of renal phosphate reabsorption. Micropuncture and microperfusion study. Pflügers Arch 369:111–118
Harvey N, Tenenhouse HS (1992) Renal Na+-phosphate cotransport in X-linked Hyp mice responds appropriately to Na+-gradient, membrane potential and pH. J Bone Miner Res 7:563–571
Knox FG, Haramati A(1985) Renal regulation of phosphate excretion. In: Seldin DW, Giebisch G (eds) The kidney : physiology and pathophysiology. Raven, New York, pp. 1381–1396
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Levi M, Lotscher M, Sorribas V, Custer M, Arar M, Kaissling B, Murer H, Biber J (1994) Cellular mechanisms of acute and chronic adaptation of rat renal phosphate transporter to alterations in dietary phosphate. Am J Physiol 267: F900-F908
Lyon MF, Scriver CR, Baker LRI, Tenenhouse HS, Kronick J, Mandla S (1986) The Gy mutation: another cause of X-linked hypophosphatemia in mouse. Proc Natl Acad Sci USA 83:4899–4903
Magagnin S, Werner A, Markovich D, Sorribas V, Stange G, Biber J, Murer H (1993) Expression cloning of human and rat renal cortex Na/Pi cotransport. Proc Natl Acad Sci USA 90:5979–5983
Meyer RA Jr, Gray RW, Meyer MH (1980) Abnormal vitamin D metabolism in the X-linked hypophosphatemic mouse. Endocrinology 107:1577–1581
Meyer RA Jr, Meyer MH, Gray RW, Bruns ME (1995) Femoral abnormalities and vitamin D metabolism in X-linked hypophosphatemic (Hyp and Gy) mice. J Orthop Res 13:30–40
Muhlbauer RC, Bonjour J, Fleisch H (1982) Abnormal tubular adaptation to dietary Pi restriction in X-linked hypophosphatemic mice. Am J Physiol 242:F353-F359
Poujeol P, Jamison RL, De Rouffignac C (1980) Phosphate reabsorption in juxtamedullary nephron terminal segments. Pflügers Arch 387:27–31
Qiu Z Q, Tenenhouse HS, Scriver CR (1993) Parental origin of mutant allele does not explain absence of gene dose in X-linked Hyp mice. Genet Res Camb 62:39–43
Scriver CR, Tenenhouse HS (1990) Conserved loci on the X chromosome confer phosphate homeostasis in mice and humans. Genet Res Camb 56:141–152
Scriver CR, Tenenhouse HS (1992) X-linked hypophosphatemia: a homologous phenotype in humans and mice with unusual organ-specific gene dosage. J Inherit Metab Dis 15:610–624
Tenenhouse HS, Jones G (1990) Abnormal regulation of renal vitamin D catabolism by dietary phosphate in murine X-linked hypophosphatemic rickets. J Clin Invest 85:1450–1455
Tenenhouse HS, Scriver CR (1979) Renal brush border membrane adaptation to phosphorus deprivation in the Hyp/Y mouse. Nature 281:225–227
Tenenhouse HS, Scriver CR, McInnes RR, Glorieux FH (1978) Renal handling of phosphate in vivo and in vitro by the X-linked hypophosphatemic male mouse: evidence for a defect in the brush border membrane. Kidney Int 14:236–244
Tenenhouse HS, Klugerman AH, Gurd W, Lapointe M, Tannenbaum GS (1988) Pituitary involvement in renal adaptation to phosphate deprivation. Am J Physiol 255:R373-R378
Tenenhouse HS, Klugerman AH, Neal JL (1989) Effect of phos-phonoformic acid, dietary phosphate and the Hyp mutation on kinetically distinct phosphate transport processes in mouse kidney. Biochim Biophys Acta 984:207–213
Tenenhouse HS, Meyer RA Jr, Mandla S, Meyer MR, Gray RW (1992) Renal phosphate transport and vitamin D metabolism in X-linked hypophosphatemic Gv mice: responses to phosphate deprivation. Endocrinology 131:51–56
Tenenhouse HS, Werner A, Biber J, Ma S, Martel J, Roy S, Murer H (1994) Renal Na+-phosphate cotransport in murine X-linked hypophosphatemic rickets: molecular characterization. J Clin Invest 93:671–676
Tenenhouse HS, Martel J, Biber J, Murer H (1995) Effect of Pi restriction on renal Na+-Pi cotransporter mRNA and immunoreactive protein in X-linked Hyp mice. Am J Physiol 268:F1062-F1069
Thornton SW, Tenenhouse HS, Martel J, Bockian RW, Meyer MR, Meyer RA (1994) X-linked hypophosphatemic Gy mice: renal tubular maximum for phosphate vs. brush-border transport after low-P diet. Am J Physiol 266:F309-F315
Author information
Authors and Affiliations
Additional information
A portion of these data has been presented in abstract form: Beck L, Meyer RA Jr, Meyer MH, Biber J, Murer H, Tenenhouse HS (1995) Effect of the X-linked Gy mutation and low phosphate diet on the renal brush-border membrane Na+-phosphate cotransport protein. J Am Soc Nephrol 6:943
Rights and permissions
About this article
Cite this article
Beck, L., Meyer, R.A., Meyer, M.H. et al. Renal expression of Na+-phosphate cotransporter mRNA and protein: Effect of the Gy mutation and low phosphate diet. Pflügers Arch. 431, 936–941 (1996). https://doi.org/10.1007/s004240050088
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s004240050088