Abstract
We monitored secretory activity of single rat melanotrophs by the patch-clamp membrane capacitance measurements (Cm). Secretory activity was stimulated by cytosol dialysis with a patch-pipette solution containing 1μM [Ca2+]i. Actin cytoskeleton was disaggregated by pretreating cells with Clostridium spiroforme toxin, which specifically ADP-ribosylates cellular actin. The extent of cytoskeleton disaggregation was monitored by phalloidin immunostaining. The maximal rate of secretion increases two folds in toxin-treated cells in comparison to controls, whereas the extent of calcium-induced secretory response was similar to that obtained in the non-treated cells. The results show that the subcortical actin network attenuates the rate of secretory activity, which we interpret to reflect a barrier function of cytoskeleton for exocytosis.
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Published: January 2000
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Chowdhury, H., Popoff, M. & Zorec, R. Actin cytoskeleton and exocytosis in rat melanotrophs. Pflügers Arch 439 (Suppl 1), r148–r149 (2000). https://doi.org/10.1007/s004240000125
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DOI: https://doi.org/10.1007/s004240000125