Exercise training reduces the risk of opportunistic infections after acute exercise and improves cytokine antigen recognition

Abstract

In general, acute exercise is thought to inhibit immune function and increase the risk of opportunistic infections, but there is some opposition to this due to a lack of quantitative evaluation. Therefore, we quantified the effect of exercise on immune function and observed the interaction between antigens and cytokines using an intramuscular infection with Trichinella spiralis (T. spiralis), a common parasitic infection model. C57BL/6 mice were used for a non-infection experiment and an infection (Inf) experiment. Each experiment was divided further into three groups: one control (CON) group, and an exercise pre-infection (PIE)-only group and exercise-sustained (ES) group, each of which was subjected to exercise for 7 weeks. All animals in the infection experiment were infected with T. spiralis 30 min after acute exercise. After infection, the ES and Inf-ES groups continued exercise for 7 additional weeks. The number of T. spiralis nurse cells remaining in skeletal muscles was fewer in the infected exercise groups compared with the infected control. Expression of interleukin-6 (IL-6) and interleukin-10 (IL-10) was higher in the Inf-CON group and transforming growth factor beta (TGF-β) expression was lower in the Inf-CON group than in the CON group, as measured by RT-PCR. In the infection experiment, only IL-10 had significant differences between the groups. Immunofluorescence revealed that most cytokines were specifically expressed around the antigenic nurse cells following exercise. In conclusion, exercise training does not increase the risk of opportunistic infections even after acute exercise, but rather reduces it. These results may be due to antigen-specific immune responses.

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Correspondence to Hee-Jae Cha or Mee Sun Ock.

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This study design was approved by the Institutional Animal Care and Use Committee of Kosin University College of Medicine (approval number: KMAP-18-13).

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This article is part of the special issue on Exercise Physiology: future opportunities and challenges in Pflügers Archiv—European Journal of Physiology

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Fig. S1

Expression of muscle cytokine gene expression. Genes encoding the IL-5, IL-6, IL-10, TNF-α and TGF-β and TGF-β cytokines were assessed by RT-PCR in three independent mice of different infection periods. All band densities were analyzed using ImageJ with GAPDH as the standard. (JPG 229 kb)

Fig. S2.

Expression of TNF-α protein. TNF-α protein levels in muscle were stained by immunofluorescence and observed for epifluorescence using a confocal laser-scanning microscope. The white arrow indicates the antigens (nurse cells). Images were captured with LSM510 software and expression level was analyzed using the imaging software (NIS-Elements). (JPG 179 kb)

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Baek, KW., Jo, JO., Kang, YJ. et al. Exercise training reduces the risk of opportunistic infections after acute exercise and improves cytokine antigen recognition. Pflugers Arch - Eur J Physiol 472, 235–244 (2020). https://doi.org/10.1007/s00424-019-02281-4

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Keywords

  • Immunity
  • Exercise
  • Cytokine
  • Infection
  • Trichinella spiralis