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L-type calcium channels in exocytosis and endocytosis of chromaffin cells

  • Carmen Nanclares
  • Andrés M. Baraibar
  • Luis GandíaEmail author
Invited Review

Abstract

The coexistence of different subtypes of voltage-dependent calcium channels (VDCC) within the same chromaffin cell (CC) and the marked interspecies variability in the proportion of VDCC subtypes that are present in the plasmalemma of the CCs raises the question on their roles in controlling different physiological functions. Particularly relevant seems to be the role of VDCCs in the regulation of the exocytotic neurotransmitter release process, and its tightly coupled membrane retrieval (endocytosis) process since both are Ca2+-dependent processes. This review is focused on the role of Ca2+ influx through L-type VDCC in the regulation of these two processes. It is currently accepted that the different VDCC subtypes (i.e., T, L, N, P/Q, R) contribute to exocytosis proportionally to their density of expression and gating properties. However, the pattern of stimulation defines a preferential role of the different subtypes of VDCC on exocytosis and endocytosis. Thus, L-type channels seem to control catecholamine release induced by prolonged stimuli while fast exocytosis in response to short square depolarizing pulses or action potentials is mediated by Ca2+ entering CCs through P/Q channels. The pattern of stimulation also influences the endocytotic process, and thus, electrophysiological data suggest the sustained Ca2+ entry through slow-inactivating L-type channels could be responsible for the activation of fast endocytosis.

Keywords

Calcium channels Exocytosis Endocytosis Chromaffin cells 

Notes

Acknowledgements

This work was partially supported by grants SAF2013-44108-P and SAF2016-78892-R (Ministerio de Economía y Competitividad, Spain) to LG. We thank the continued support of Fundación Teófilo Hernando, Madrid, Spain.

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© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Instituto Teófilo Hernando, Departamento de Farmacología y Terapéutica, Facultad de MedicinaUniversidad Autónoma de MadridMadridSpain

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