Erratum to: Pflugers Arch - Eur J Physiol

DOI 10.1007/s00424-016-1894-6

The original publication of this paper contains a mistake.

The correct image of figure 3 is shown below:

Fig. 3
figure 1

Counter-flow experiments on OAT3. a Time course of ES efflux of OAT3- and pcDNA5-transfected HEK293 cells in the absence (filled circle) and presence of 0.25 mM ES (unfilled circle) and 0.25 mM dantrolene (filled triangle). OAT3 (filled circle, unfilled circle, filled triangle)- and pcDNA5 (unfilled triangle)-transfected HEK293 cells were incubated for 60 min at 37 °C in solutions containing 10 nM [3H]ES. After a wash-out, cells were incubated in 10 nM [3H]ES minus or plus un-labeled 0.25 mM ES or dantrolene. At the time indicated, the [3H]ES content of the cells was measured. b Specificity of counter-flow. Cells were incubated for 60 min at 37 °C in solutions containing 10 nM [3H]ES. Afterwards, cells were incubated for 10 min in solutions containing 10 nM [3H]ES either in the absence (control) or presence of the indicated compounds. After wash-out in ice-cold PBS, the [3H]ES content was determined. ES, dantrolene, 5-OH dantrolene, ES, and glutarate, each to a different extent, stimulated loss of ES, whereas succinate did not reduce ES content. ES content of pcDNA5-transfected cells was unaffected by the above treatments. c Counter-flow induced by known OAT3 substrates. Experiments were performed as described in b. Concentrations of bumetanide and furosemide were 0.25 mM each; experiments presented in a–c were performed in triplicate on consecutive cell passages. d Endogenous mRNA expression of BCRP in HEK293 cells. mRNA coding for BCRP was analyzed in non-transfected HEK293 cells by real-time PCR. As a positive control, OAT3 mRNA from OAT3-transfected HEK293 cells was used. Data are means ± SE and expressed as ΔCt values. Negative bars (BCRP) indicate lower, positive bars (OAT3) indicate higher mRNA content as compared to the housekeeping genes (HPRT1, GAPDH)

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