Orexinergic system in the locus coeruleus modulates the CO2 ventilatory response

  • Mariane C. Vicente
  • Mirela B. Dias
  • Elisa M. Fonseca
  • Kênia C. Bícego
  • Luciane H. GargaglioniEmail author
Integrative physiology


The orexins are hypothalamic neuropeptides involved in an array of functions such as regulation of sleep/wake states and chemoreception to CO2/pH. The locus coeruleus (LC) is a chemosensitive site and expresses an extensive population of orexin receptor 1 (OX1R). We tested the hypothesis that OX1Rs located in the LC participate in the ventilatory response to hypercapnia in a vigilance state and diurnal cycle-dependent manner. For this, we performed unilateral injections of SB-334867 (OX1R antagonist, 5 mM) into the LC of male Wistar rats and evaluated the ventilatory response to 7 % CO2 during wakefulness and sleep in the dark and light phases of the diurnal cycle. Hypercapnia induced an increase in ventilation (V E) in all groups compared to normocapnic values. However, during the dark phase, but not in the light phase, SB-334867 injection promoted an attenuation of the hypercapnic chemoreflex during wakefulness (V E: vehicle, 1502.6 ± 100 mL kg−1 min−1 vs SB-334867, 1200.3 ± 70.0 mL kg−1 min−1) but not during sleep (V E: vehicle, 1383.0 ± 113.9 vs SB-334687, 1287.6 ± 92.1 mL kg−1 min−1), due to changes in tidal volume (V T). We suggest that projections of orexin-containing neurons to the LC contribute, via OX1Rs, to the hypercapnic chemoreflex during wakefulness in the dark phase.


Awake Chemoreception Orexin Hypercapnia Sleep Ventilation 



This work was supported by Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP - 2012/19966-0), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq - 442560/2014-1). The authors thank Euclides Seccato for his technical assistance and Luis Gustavo Patrone for helping with the statistics.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.


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© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Mariane C. Vicente
    • 1
  • Mirela B. Dias
    • 2
  • Elisa M. Fonseca
    • 1
  • Kênia C. Bícego
    • 1
  • Luciane H. Gargaglioni
    • 1
    Email author
  1. 1.Department of Animal Morphology and PhysiologySao Paulo State University—UNESP/FCAV at JaboticabalJaboticabalBrazil
  2. 2.Department of Physiology, Institute of BioscienceSão Paulo State University—UNESPBotucatuBrazil

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